Subcellular organelle lipidomics in TLR-4-activated macrophages

Alexander Y. Andreyev, Eoin Fahy, Ziqiang Guan, Samuel Kelly, Xiang Li, Jeffrey G. McDonald, Stephen Milne, David Myers, Hyejung Park, Andrea Ryan, Bonne M. Thompson, Elaine Wang, Yihua Zhao, H. Alex Brown, Alfred H. Merrill, Christian R H Raetz, David W. Russell, Shankar Subramaniam, Edward A. Dennis

Research output: Contribution to journalArticle

122 Citations (Scopus)

Abstract

Lipids orchestrate biological processes by acting remotely as signaling molecules or locally as membrane components that modulate protein function. Detailed insight into lipid function requires knowledge of the subcellular localization of individual lipids. We report an analysis of the subcellular lipidome of the mammalian macrophage, a cell type that plays key roles in inflammation, immune responses, and phagocytosis. Nuclei, mitochondria, endoplasmic reticulum (ER), plasmalemma, and cytoplasm were isolated from RAW 264.7 macrophages in basal and activated states. Subsequent lipidomic analyses of major membrane lipid categories identified 229 individual/isobaric species, including 163 glycerophospholipids, 48 sphingolipids, 13 sterols, and 5 prenols. Major subcellular compartments exhibited substantially divergent glycerophospholipid profiles. Activation of macrophages by the Toll-like receptor 4-specific lipopolysaccharide Kdo2-lipid A caused significant remodeling of the subcellular lipidome. Some changes in lipid composition occurred in all compartments (e.g., increases in the levels of ceramides and the cholesterol precursors desmosterol and lanosterol). Other changes were manifest in specific organelles. For example, oxidized sterols increased and unsaturated cardiolipins decreased in mitochondria, whereas unsaturated ether-linked phosphatidylethanolamines decreased in the ER. We speculate that these changes may reflect mitochondrial oxidative stress and the release of arachidonic acid from the ER in response to cell activation.

Original languageEnglish (US)
Pages (from-to)2785-2797
Number of pages13
JournalJournal of Lipid Research
Volume51
Issue number9
DOIs
StatePublished - Sep 2010

Fingerprint

Macrophages
Organelles
Endoplasmic Reticulum
Glycerophospholipids
Lipids
Mitochondria
Sterols
Desmosterol
Chemical activation
Lanosterol
Phosphatidylethanolamines
Biological Phenomena
Sphingolipids
Toll-Like Receptor 4
Cardiolipins
Oxidative stress
Macrophage Activation
Ceramides
Membrane Lipids
Phagocytosis

Keywords

  • Endoplasmic reticulum
  • Lipidome
  • Lipopolysaccharide
  • Membrane
  • Mitochondria
  • Nucleus
  • Plasmalemma

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Endocrinology

Cite this

Andreyev, A. Y., Fahy, E., Guan, Z., Kelly, S., Li, X., McDonald, J. G., ... Dennis, E. A. (2010). Subcellular organelle lipidomics in TLR-4-activated macrophages. Journal of Lipid Research, 51(9), 2785-2797. https://doi.org/10.1194/jlr.M008748

Subcellular organelle lipidomics in TLR-4-activated macrophages. / Andreyev, Alexander Y.; Fahy, Eoin; Guan, Ziqiang; Kelly, Samuel; Li, Xiang; McDonald, Jeffrey G.; Milne, Stephen; Myers, David; Park, Hyejung; Ryan, Andrea; Thompson, Bonne M.; Wang, Elaine; Zhao, Yihua; Brown, H. Alex; Merrill, Alfred H.; Raetz, Christian R H; Russell, David W.; Subramaniam, Shankar; Dennis, Edward A.

In: Journal of Lipid Research, Vol. 51, No. 9, 09.2010, p. 2785-2797.

Research output: Contribution to journalArticle

Andreyev, AY, Fahy, E, Guan, Z, Kelly, S, Li, X, McDonald, JG, Milne, S, Myers, D, Park, H, Ryan, A, Thompson, BM, Wang, E, Zhao, Y, Brown, HA, Merrill, AH, Raetz, CRH, Russell, DW, Subramaniam, S & Dennis, EA 2010, 'Subcellular organelle lipidomics in TLR-4-activated macrophages', Journal of Lipid Research, vol. 51, no. 9, pp. 2785-2797. https://doi.org/10.1194/jlr.M008748
Andreyev, Alexander Y. ; Fahy, Eoin ; Guan, Ziqiang ; Kelly, Samuel ; Li, Xiang ; McDonald, Jeffrey G. ; Milne, Stephen ; Myers, David ; Park, Hyejung ; Ryan, Andrea ; Thompson, Bonne M. ; Wang, Elaine ; Zhao, Yihua ; Brown, H. Alex ; Merrill, Alfred H. ; Raetz, Christian R H ; Russell, David W. ; Subramaniam, Shankar ; Dennis, Edward A. / Subcellular organelle lipidomics in TLR-4-activated macrophages. In: Journal of Lipid Research. 2010 ; Vol. 51, No. 9. pp. 2785-2797.
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