Substance P-like immunoreactivity in the suprachiasmatic nucleus of the rat

Yasumasa Otori, Keiko Tominaga, Chiaki Fukuhara, Jing Yang, Shin Yamazaki, Felino Ramon A Cagampang, Hitoshi Okamura, Shin Ichi T Inouye

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The content of substance P (SP)-like immunoreactivity (LI) within the suprachiasmatic nucleus (SCN) of rats was determined by enzyme immunoassay to evaluate the effect of light on SP-LI in the rat SCN. Male rats were kept under various lighting conditions: light-dark cycles, constant darkness, continuous light exposure for 24 h or light pulse interrupting constant darkness. Animals were also subjected to ocular enucleation. The present study showed that SP-LI in the SCN was unaffected by environmental lighting conditions or by bilateral ocular enucleation. Immunohistochemical studies also confirmed that SP immunoreactivity, which was found in the ventrolateral (VL) subdivision of the SCN, was not reduced significantly even after ocular enucleation. These results suggest that, in contrast to other neurotransmitters in the VL portion of the SCN such as vasoactive intestinal polypeptide (VIP), gastrin releasing peptide (GRP) and neuropeptide Y (NPY), SP level in the SCN is quite stable to light and arises from an area other than the retina.

Original languageEnglish (US)
Pages (from-to)271-277
Number of pages7
JournalBrain Research
Volume619
Issue number1-2
DOIs
StatePublished - Aug 13 1993

Fingerprint

Suprachiasmatic Nucleus
Substance P
Eye Enucleation
Light
Darkness
Lighting
Gastrin-Releasing Peptide
Neuropeptide Y
Vasoactive Intestinal Peptide
Photoperiod
Immunoenzyme Techniques
Neurotransmitter Agents
Retina

Keywords

  • Circadian rhythm
  • Enzyme immunoassay
  • Rat
  • Retinohypothalamic tract
  • Substance P
  • Suprachiasmatic nucleus

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Otori, Y., Tominaga, K., Fukuhara, C., Yang, J., Yamazaki, S., Cagampang, F. R. A., ... Inouye, S. I. T. (1993). Substance P-like immunoreactivity in the suprachiasmatic nucleus of the rat. Brain Research, 619(1-2), 271-277. https://doi.org/10.1016/0006-8993(93)91621-X

Substance P-like immunoreactivity in the suprachiasmatic nucleus of the rat. / Otori, Yasumasa; Tominaga, Keiko; Fukuhara, Chiaki; Yang, Jing; Yamazaki, Shin; Cagampang, Felino Ramon A; Okamura, Hitoshi; Inouye, Shin Ichi T.

In: Brain Research, Vol. 619, No. 1-2, 13.08.1993, p. 271-277.

Research output: Contribution to journalArticle

Otori, Y, Tominaga, K, Fukuhara, C, Yang, J, Yamazaki, S, Cagampang, FRA, Okamura, H & Inouye, SIT 1993, 'Substance P-like immunoreactivity in the suprachiasmatic nucleus of the rat', Brain Research, vol. 619, no. 1-2, pp. 271-277. https://doi.org/10.1016/0006-8993(93)91621-X
Otori, Yasumasa ; Tominaga, Keiko ; Fukuhara, Chiaki ; Yang, Jing ; Yamazaki, Shin ; Cagampang, Felino Ramon A ; Okamura, Hitoshi ; Inouye, Shin Ichi T. / Substance P-like immunoreactivity in the suprachiasmatic nucleus of the rat. In: Brain Research. 1993 ; Vol. 619, No. 1-2. pp. 271-277.
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