Substance P-like immunoreactivity in the suprachiasmatic nucleus of the rat

Yasumasa Otori; Keiko Tominaga; Chiaki Fukuhara; Jing Yang; Shin Yamazaki; Felino Ramon A Cagampang; Hitoshi Okamura; Shin Ichi T Inouye

doi:10.1016/0006-8993(93)91621-X

Substance P-like immunoreactivity in the suprachiasmatic nucleus of the rat

Yasumasa Otori, Keiko Tominaga, Chiaki Fukuhara, Jing Yang, Shin Yamazaki, Felino Ramon A Cagampang, Hitoshi Okamura, Shin Ichi T Inouye

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Abstract

The content of substance P (SP)-like immunoreactivity (LI) within the suprachiasmatic nucleus (SCN) of rats was determined by enzyme immunoassay to evaluate the effect of light on SP-LI in the rat SCN. Male rats were kept under various lighting conditions: light-dark cycles, constant darkness, continuous light exposure for 24 h or light pulse interrupting constant darkness. Animals were also subjected to ocular enucleation. The present study showed that SP-LI in the SCN was unaffected by environmental lighting conditions or by bilateral ocular enucleation. Immunohistochemical studies also confirmed that SP immunoreactivity, which was found in the ventrolateral (VL) subdivision of the SCN, was not reduced significantly even after ocular enucleation. These results suggest that, in contrast to other neurotransmitters in the VL portion of the SCN such as vasoactive intestinal polypeptide (VIP), gastrin releasing peptide (GRP) and neuropeptide Y (NPY), SP level in the SCN is quite stable to light and arises from an area other than the retina.

Original language	English (US)
Pages (from-to)	271-277
Number of pages	7
Journal	Brain Research
Volume	619
Issue number	1-2
DOIs	https://doi.org/10.1016/0006-8993(93)91621-X
State	Published - Aug 13 1993

Keywords

Circadian rhythm
Enzyme immunoassay
Rat
Retinohypothalamic tract
Substance P
Suprachiasmatic nucleus

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(93)91621-X

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title = "Substance P-like immunoreactivity in the suprachiasmatic nucleus of the rat",

abstract = "The content of substance P (SP)-like immunoreactivity (LI) within the suprachiasmatic nucleus (SCN) of rats was determined by enzyme immunoassay to evaluate the effect of light on SP-LI in the rat SCN. Male rats were kept under various lighting conditions: light-dark cycles, constant darkness, continuous light exposure for 24 h or light pulse interrupting constant darkness. Animals were also subjected to ocular enucleation. The present study showed that SP-LI in the SCN was unaffected by environmental lighting conditions or by bilateral ocular enucleation. Immunohistochemical studies also confirmed that SP immunoreactivity, which was found in the ventrolateral (VL) subdivision of the SCN, was not reduced significantly even after ocular enucleation. These results suggest that, in contrast to other neurotransmitters in the VL portion of the SCN such as vasoactive intestinal polypeptide (VIP), gastrin releasing peptide (GRP) and neuropeptide Y (NPY), SP level in the SCN is quite stable to light and arises from an area other than the retina.",

keywords = "Circadian rhythm, Enzyme immunoassay, Rat, Retinohypothalamic tract, Substance P, Suprachiasmatic nucleus",

author = "Yasumasa Otori and Keiko Tominaga and Chiaki Fukuhara and Jing Yang and Shin Yamazaki and Cagampang, {Felino Ramon A} and Hitoshi Okamura and Inouye, {Shin Ichi T}",

note = "Funding Information: Acknowledgements. The authors are grateful to Dr. Takehiko Amano from Tokai University for supplying antiserum raised against substance P. The authors wish to thank Miss Ako Tokumasu for her technical assistance. These experiments were supported by Mit-subishi Kasei Institute of Life Sciences Grant 'Project 10'.",

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doi = "10.1016/0006-8993(93)91621-X",

language = "English (US)",

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pages = "271--277",

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number = "1-2",

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T1 - Substance P-like immunoreactivity in the suprachiasmatic nucleus of the rat

AU - Otori, Yasumasa

AU - Tominaga, Keiko

AU - Fukuhara, Chiaki

AU - Yang, Jing

AU - Yamazaki, Shin

AU - Cagampang, Felino Ramon A

AU - Okamura, Hitoshi

AU - Inouye, Shin Ichi T

N1 - Funding Information: Acknowledgements. The authors are grateful to Dr. Takehiko Amano from Tokai University for supplying antiserum raised against substance P. The authors wish to thank Miss Ako Tokumasu for her technical assistance. These experiments were supported by Mit-subishi Kasei Institute of Life Sciences Grant 'Project 10'.

PY - 1993/8/13

Y1 - 1993/8/13

N2 - The content of substance P (SP)-like immunoreactivity (LI) within the suprachiasmatic nucleus (SCN) of rats was determined by enzyme immunoassay to evaluate the effect of light on SP-LI in the rat SCN. Male rats were kept under various lighting conditions: light-dark cycles, constant darkness, continuous light exposure for 24 h or light pulse interrupting constant darkness. Animals were also subjected to ocular enucleation. The present study showed that SP-LI in the SCN was unaffected by environmental lighting conditions or by bilateral ocular enucleation. Immunohistochemical studies also confirmed that SP immunoreactivity, which was found in the ventrolateral (VL) subdivision of the SCN, was not reduced significantly even after ocular enucleation. These results suggest that, in contrast to other neurotransmitters in the VL portion of the SCN such as vasoactive intestinal polypeptide (VIP), gastrin releasing peptide (GRP) and neuropeptide Y (NPY), SP level in the SCN is quite stable to light and arises from an area other than the retina.

AB - The content of substance P (SP)-like immunoreactivity (LI) within the suprachiasmatic nucleus (SCN) of rats was determined by enzyme immunoassay to evaluate the effect of light on SP-LI in the rat SCN. Male rats were kept under various lighting conditions: light-dark cycles, constant darkness, continuous light exposure for 24 h or light pulse interrupting constant darkness. Animals were also subjected to ocular enucleation. The present study showed that SP-LI in the SCN was unaffected by environmental lighting conditions or by bilateral ocular enucleation. Immunohistochemical studies also confirmed that SP immunoreactivity, which was found in the ventrolateral (VL) subdivision of the SCN, was not reduced significantly even after ocular enucleation. These results suggest that, in contrast to other neurotransmitters in the VL portion of the SCN such as vasoactive intestinal polypeptide (VIP), gastrin releasing peptide (GRP) and neuropeptide Y (NPY), SP level in the SCN is quite stable to light and arises from an area other than the retina.

KW - Circadian rhythm

KW - Enzyme immunoassay

KW - Rat

KW - Retinohypothalamic tract

KW - Substance P

KW - Suprachiasmatic nucleus

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Substance P-like immunoreactivity in the suprachiasmatic nucleus of the rat

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Keywords

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