Substrate selection early after reperfusion of ischemic regions in the working rabbit heart

Michael A. Solomon, F. Mark H Jeffrey, Charles J. Storey, A. Dean Sherry, Craig R. Malloy

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Several substrates are available in vivo for oxidation by the myocardium. Although substrate selection has been studied extensively in normoxic myocardium, relatively little is known about substrate preference very early during reperfusion after ischemia. Carbon-13 isotopomer analysis was used to study substrate usage by nonischemic and reperfused-ischemic myocardium in a working heart that was subjected to 15 min of regional ischemia and reperfused for 5 min. Compared with nonischemic myocardium, the contribution of acetoacetate to acetyl coenzyme A was increased in the reperfused-ischemic region, and the contribution of exogenous lactate was decreased. Free fatty acid oxidation, however, was not different in the two regions. These results indicate that (1) early during reperfusion, ketone body oxidation may be more significant than has been emphasized, (2) the relative contribution of fatty acids to acetyl coenzyme A is not sensitive to ischemia followed by reperfusion, and (3) Carbon-13 magnetic resonance spectroscopy methods may be used for analysis of spatial heterogeneity of metabolism in the heart.

Original languageEnglish (US)
Pages (from-to)820-826
Number of pages7
JournalMagnetic resonance in medicine
Volume35
Issue number6
DOIs
StatePublished - Jun 1996

Keywords

  • C MRS
  • Myocardial metabolism
  • isotopomer
  • reperfusion

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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