Subunit interactions in yeast transcription/repair factor TFIIH. Requirement for Tfb3 subunit in nucleotide excision repair

William J. Feaver, Wenya Huang, Opher Gileadi, Lawrence Myers, Claes M. Gustafsson, Roger D. Kornberg, Errol C. Friedberg

Research output: Contribution to journalArticle

21 Scopus citations


A yeast strain harboring a temperature-sensitive allele of TFB3 (tfb3(ts)), the 38-kDa subunit of the RNA polymerase II transcription/nucleotide excision repair factor TFIIH, was found to be sensitive to ultraviolet (UV) radiation and defective for nucleotide excision repair in vitro. Interestingly, tfb3(ts) failed to grow on medium containing caffeine. A comprehensive pairwise two-hybrid analysis between yeast TFIIH subunits identified novel interactions between Rad3 and Tfb3, Tfb4 and Ssl1, as well as Ssl2 and Tfb2. These interactions have facilitated a more complete model of the structure of TFIIH and the nucleotide excision repairosome.

Original languageEnglish (US)
Pages (from-to)5941-5946
Number of pages6
JournalJournal of Biological Chemistry
Issue number8
StatePublished - Feb 25 2000


ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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