TY - JOUR
T1 - Subunit interactions in yeast transcription/repair factor TFIIH. Requirement for Tfb3 subunit in nucleotide excision repair
AU - Feaver, William J.
AU - Huang, Wenya
AU - Gileadi, Opher
AU - Myers, Lawrence
AU - Gustafsson, Claes M.
AU - Kornberg, Roger D.
AU - Friedberg, Errol C.
PY - 2000/2/25
Y1 - 2000/2/25
N2 - A yeast strain harboring a temperature-sensitive allele of TFB3 (tfb3(ts)), the 38-kDa subunit of the RNA polymerase II transcription/nucleotide excision repair factor TFIIH, was found to be sensitive to ultraviolet (UV) radiation and defective for nucleotide excision repair in vitro. Interestingly, tfb3(ts) failed to grow on medium containing caffeine. A comprehensive pairwise two-hybrid analysis between yeast TFIIH subunits identified novel interactions between Rad3 and Tfb3, Tfb4 and Ssl1, as well as Ssl2 and Tfb2. These interactions have facilitated a more complete model of the structure of TFIIH and the nucleotide excision repairosome.
AB - A yeast strain harboring a temperature-sensitive allele of TFB3 (tfb3(ts)), the 38-kDa subunit of the RNA polymerase II transcription/nucleotide excision repair factor TFIIH, was found to be sensitive to ultraviolet (UV) radiation and defective for nucleotide excision repair in vitro. Interestingly, tfb3(ts) failed to grow on medium containing caffeine. A comprehensive pairwise two-hybrid analysis between yeast TFIIH subunits identified novel interactions between Rad3 and Tfb3, Tfb4 and Ssl1, as well as Ssl2 and Tfb2. These interactions have facilitated a more complete model of the structure of TFIIH and the nucleotide excision repairosome.
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U2 - 10.1074/jbc.275.8.5941
DO - 10.1074/jbc.275.8.5941
M3 - Article
C2 - 10681587
AN - SCOPUS:0034050729
SN - 0021-9258
VL - 275
SP - 5941
EP - 5946
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 8
ER -