Successful elimination of memory-type CD8+ T cell subsets by the administration of anti-Gr-1 monoclonal antibody in vivo

Junko Matsuzaki, Takemasa Tsuji, Kenji Chamoto, Tsuguhide Takeshima, Fujiro Sendo, Takashi Nishimura

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

During investigating the expression of Gr-1 antigen on various subsets of mouse spleen cells, we found that Gr-1 was expressed on memory-type CD8 +CD44highCD62Lhigh T cells in addition to granulocytes. Intraperitoneal administration of anti-Gr-1 mAb caused almost complete elimination of Ly-6C+ memory-type CD8+ T cells as well as Ly-6G+ granulocytes. Anti-Gr-1 mAb-treated mouse spleen cells exhibited greatly reduced IFN-γ production in response to anti-CD3 mAb both in vitro and in vivo. This reduced cytokine production appeared to be derived from elimination of IFN-γ-producing Gr-1+CD8 + T cells. Indeed, CD8+ T cells with IFN-γ- producing activity and cytotoxicity were generated from isolated Gr-1 +CD8+ cells but not from Gr-1-CD8+ T cells. We also demonstrated that therapeutic effect of MBL-2 tumor-immunized spleen cells was greatly reduced by anti-Gr-1 mAb-treatment. Thus, we initially demonstrated that anti-Gr-1 mAb might become a good tool to investigate a precise role for memory-type CD8+ T cells in vivo.

Original languageEnglish (US)
Pages (from-to)98-105
Number of pages8
JournalCellular Immunology
Volume224
Issue number2
DOIs
StatePublished - Aug 2003

Keywords

  • Antitumor activity
  • CD8 T cell
  • Gr-1
  • IFN-γ
  • Memory-type

ASJC Scopus subject areas

  • Immunology

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