Abstract
During investigating the expression of Gr-1 antigen on various subsets of mouse spleen cells, we found that Gr-1 was expressed on memory-type CD8 +CD44highCD62Lhigh T cells in addition to granulocytes. Intraperitoneal administration of anti-Gr-1 mAb caused almost complete elimination of Ly-6C+ memory-type CD8+ T cells as well as Ly-6G+ granulocytes. Anti-Gr-1 mAb-treated mouse spleen cells exhibited greatly reduced IFN-γ production in response to anti-CD3 mAb both in vitro and in vivo. This reduced cytokine production appeared to be derived from elimination of IFN-γ-producing Gr-1+CD8 + T cells. Indeed, CD8+ T cells with IFN-γ- producing activity and cytotoxicity were generated from isolated Gr-1 +CD8+ cells but not from Gr-1-CD8+ T cells. We also demonstrated that therapeutic effect of MBL-2 tumor-immunized spleen cells was greatly reduced by anti-Gr-1 mAb-treatment. Thus, we initially demonstrated that anti-Gr-1 mAb might become a good tool to investigate a precise role for memory-type CD8+ T cells in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 97-104 |
| Number of pages | 8 |
| Journal | Cellular Immunology |
| Volume | 224 |
| Issue number | 2 |
| DOIs | |
| State | Published - Aug 2003 |
Fingerprint
Keywords
- Antitumor activity
- CD8 T cell
- Gr-1
- IFN-γ
- Memory-type
ASJC Scopus subject areas
- Cell Biology
- Immunology
Cite this
Successful elimination of memory-type CD8+ T cell subsets by the administration of anti-Gr-1 monoclonal antibody in vivo. / Matsuzaki, Junko; Tsuji, Takemasa; Chamoto, Kenji; Takeshima, Tsuguhide; Sendo, Fujiro; Nishimura, Takashi.
In: Cellular Immunology, Vol. 224, No. 2, 08.2003, p. 97-104.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Successful elimination of memory-type CD8+ T cell subsets by the administration of anti-Gr-1 monoclonal antibody in vivo
AU - Matsuzaki, Junko
AU - Tsuji, Takemasa
AU - Chamoto, Kenji
AU - Takeshima, Tsuguhide
AU - Sendo, Fujiro
AU - Nishimura, Takashi
PY - 2003/8
Y1 - 2003/8
N2 - During investigating the expression of Gr-1 antigen on various subsets of mouse spleen cells, we found that Gr-1 was expressed on memory-type CD8 +CD44highCD62Lhigh T cells in addition to granulocytes. Intraperitoneal administration of anti-Gr-1 mAb caused almost complete elimination of Ly-6C+ memory-type CD8+ T cells as well as Ly-6G+ granulocytes. Anti-Gr-1 mAb-treated mouse spleen cells exhibited greatly reduced IFN-γ production in response to anti-CD3 mAb both in vitro and in vivo. This reduced cytokine production appeared to be derived from elimination of IFN-γ-producing Gr-1+CD8 + T cells. Indeed, CD8+ T cells with IFN-γ- producing activity and cytotoxicity were generated from isolated Gr-1 +CD8+ cells but not from Gr-1-CD8+ T cells. We also demonstrated that therapeutic effect of MBL-2 tumor-immunized spleen cells was greatly reduced by anti-Gr-1 mAb-treatment. Thus, we initially demonstrated that anti-Gr-1 mAb might become a good tool to investigate a precise role for memory-type CD8+ T cells in vivo.
AB - During investigating the expression of Gr-1 antigen on various subsets of mouse spleen cells, we found that Gr-1 was expressed on memory-type CD8 +CD44highCD62Lhigh T cells in addition to granulocytes. Intraperitoneal administration of anti-Gr-1 mAb caused almost complete elimination of Ly-6C+ memory-type CD8+ T cells as well as Ly-6G+ granulocytes. Anti-Gr-1 mAb-treated mouse spleen cells exhibited greatly reduced IFN-γ production in response to anti-CD3 mAb both in vitro and in vivo. This reduced cytokine production appeared to be derived from elimination of IFN-γ-producing Gr-1+CD8 + T cells. Indeed, CD8+ T cells with IFN-γ- producing activity and cytotoxicity were generated from isolated Gr-1 +CD8+ cells but not from Gr-1-CD8+ T cells. We also demonstrated that therapeutic effect of MBL-2 tumor-immunized spleen cells was greatly reduced by anti-Gr-1 mAb-treatment. Thus, we initially demonstrated that anti-Gr-1 mAb might become a good tool to investigate a precise role for memory-type CD8+ T cells in vivo.
KW - Antitumor activity
KW - CD8 T cell
KW - Gr-1
KW - IFN-γ
KW - Memory-type
UR - http://www.scopus.com/inward/record.url?scp=0242333943&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0242333943&partnerID=8YFLogxK
U2 - 10.1016/j.cellimm.2003.08.009
DO - 10.1016/j.cellimm.2003.08.009
M3 - Article
C2 - 14609575
AN - SCOPUS:0242333943
VL - 224
SP - 97
EP - 104
JO - Cellular Immunology
JF - Cellular Immunology
SN - 0008-8749
IS - 2
ER -