Successful immunization against Acanthamoeba keratitis in a pig model

H. Alizadeh, Y. He, J. P. McCulley, D. Ma, G. L. Stewart, M. Via, E. Haehling, J. Y. Niederkorn

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The feasibility of inducing protective immunity to Acanthamoeba keratitis was tested in a pig model. Experiments were designed to determine if ocular infection with Acanthamoeba trophozoites would elicit protection against reinfection. Additional experiments examined whether injection of parasite antigens either intramuscularly, subconjunctivally, or by both routes would induce immunity. Therefore, four groups of animals were examined: (a) pigs that had resolved a primary corneal infection with Acanthamoeba; (b) pigs immunized intramuscularly; (c) pigs immunized subconjunctivally; and (d) pigs immunized intramuscularly and subconjunctivally. Animals were subsequently challenged with parasite-laden soft contact lenses and observed clinically for the appearance of Acanthamoeba keratitis. Acanthamoeba-specific serum antibody titers and blastogenic responses of peripheral blood lymphocytes were determined weekly. The results indicated that intramuscular injection of Acanthamoeba antigens failed to protect against ocular infection even though hosts developed high liters of IgG antibodies and displayed lymphocyte blastogenic responses to parasite antigens. Ocular infection alone failed to stimulate immunity in any of the animals. By contrast, 50% of the hosts immunized subconjunctivally were protected against corneal disease, and 100% of the animals immunized by a combination of intramuscular and sub- conjunctival administration of parasite antigens were completely protected against two separate ocular challenges with infectious parasites. Protection did not correlate with either IgG antibody titers or blastogenic potentials of peripheral blood lymphocytes. Interestingly, ocular infection alone failed to stimulate immunity to subsequent ocular challenge with infectious parasites. Thus, administration of parasite antigen via the subconjunctival route can protect against Acanthamoeba keratitis.

Original languageEnglish (US)
Pages (from-to)180-186
Number of pages7
JournalCornea
Volume14
Issue number2
StatePublished - 1995

Fingerprint

Acanthamoeba Keratitis
Immunization
Parasites
Swine
Acanthamoeba
Eye Infections
Immunity
Antigens
Lymphocytes
Antibodies
Immunoglobulin G
Corneal Diseases
Hydrophilic Contact Lens
Trophozoites
Intramuscular Injections
Injections

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Successful immunization against Acanthamoeba keratitis in a pig model. / Alizadeh, H.; He, Y.; McCulley, J. P.; Ma, D.; Stewart, G. L.; Via, M.; Haehling, E.; Niederkorn, J. Y.

In: Cornea, Vol. 14, No. 2, 1995, p. 180-186.

Research output: Contribution to journalArticle

Alizadeh, H, He, Y, McCulley, JP, Ma, D, Stewart, GL, Via, M, Haehling, E & Niederkorn, JY 1995, 'Successful immunization against Acanthamoeba keratitis in a pig model', Cornea, vol. 14, no. 2, pp. 180-186.
Alizadeh H, He Y, McCulley JP, Ma D, Stewart GL, Via M et al. Successful immunization against Acanthamoeba keratitis in a pig model. Cornea. 1995;14(2):180-186.
Alizadeh, H. ; He, Y. ; McCulley, J. P. ; Ma, D. ; Stewart, G. L. ; Via, M. ; Haehling, E. ; Niederkorn, J. Y. / Successful immunization against Acanthamoeba keratitis in a pig model. In: Cornea. 1995 ; Vol. 14, No. 2. pp. 180-186.
@article{bb829c1d3fae4491a313f28558c81e0c,
title = "Successful immunization against Acanthamoeba keratitis in a pig model",
abstract = "The feasibility of inducing protective immunity to Acanthamoeba keratitis was tested in a pig model. Experiments were designed to determine if ocular infection with Acanthamoeba trophozoites would elicit protection against reinfection. Additional experiments examined whether injection of parasite antigens either intramuscularly, subconjunctivally, or by both routes would induce immunity. Therefore, four groups of animals were examined: (a) pigs that had resolved a primary corneal infection with Acanthamoeba; (b) pigs immunized intramuscularly; (c) pigs immunized subconjunctivally; and (d) pigs immunized intramuscularly and subconjunctivally. Animals were subsequently challenged with parasite-laden soft contact lenses and observed clinically for the appearance of Acanthamoeba keratitis. Acanthamoeba-specific serum antibody titers and blastogenic responses of peripheral blood lymphocytes were determined weekly. The results indicated that intramuscular injection of Acanthamoeba antigens failed to protect against ocular infection even though hosts developed high liters of IgG antibodies and displayed lymphocyte blastogenic responses to parasite antigens. Ocular infection alone failed to stimulate immunity in any of the animals. By contrast, 50{\%} of the hosts immunized subconjunctivally were protected against corneal disease, and 100{\%} of the animals immunized by a combination of intramuscular and sub- conjunctival administration of parasite antigens were completely protected against two separate ocular challenges with infectious parasites. Protection did not correlate with either IgG antibody titers or blastogenic potentials of peripheral blood lymphocytes. Interestingly, ocular infection alone failed to stimulate immunity to subsequent ocular challenge with infectious parasites. Thus, administration of parasite antigen via the subconjunctival route can protect against Acanthamoeba keratitis.",
author = "H. Alizadeh and Y. He and McCulley, {J. P.} and D. Ma and Stewart, {G. L.} and M. Via and E. Haehling and Niederkorn, {J. Y.}",
year = "1995",
language = "English (US)",
volume = "14",
pages = "180--186",
journal = "Cornea",
issn = "0277-3740",
publisher = "Lippincott Williams and Wilkins",
number = "2",

}

TY - JOUR

T1 - Successful immunization against Acanthamoeba keratitis in a pig model

AU - Alizadeh, H.

AU - He, Y.

AU - McCulley, J. P.

AU - Ma, D.

AU - Stewart, G. L.

AU - Via, M.

AU - Haehling, E.

AU - Niederkorn, J. Y.

PY - 1995

Y1 - 1995

N2 - The feasibility of inducing protective immunity to Acanthamoeba keratitis was tested in a pig model. Experiments were designed to determine if ocular infection with Acanthamoeba trophozoites would elicit protection against reinfection. Additional experiments examined whether injection of parasite antigens either intramuscularly, subconjunctivally, or by both routes would induce immunity. Therefore, four groups of animals were examined: (a) pigs that had resolved a primary corneal infection with Acanthamoeba; (b) pigs immunized intramuscularly; (c) pigs immunized subconjunctivally; and (d) pigs immunized intramuscularly and subconjunctivally. Animals were subsequently challenged with parasite-laden soft contact lenses and observed clinically for the appearance of Acanthamoeba keratitis. Acanthamoeba-specific serum antibody titers and blastogenic responses of peripheral blood lymphocytes were determined weekly. The results indicated that intramuscular injection of Acanthamoeba antigens failed to protect against ocular infection even though hosts developed high liters of IgG antibodies and displayed lymphocyte blastogenic responses to parasite antigens. Ocular infection alone failed to stimulate immunity in any of the animals. By contrast, 50% of the hosts immunized subconjunctivally were protected against corneal disease, and 100% of the animals immunized by a combination of intramuscular and sub- conjunctival administration of parasite antigens were completely protected against two separate ocular challenges with infectious parasites. Protection did not correlate with either IgG antibody titers or blastogenic potentials of peripheral blood lymphocytes. Interestingly, ocular infection alone failed to stimulate immunity to subsequent ocular challenge with infectious parasites. Thus, administration of parasite antigen via the subconjunctival route can protect against Acanthamoeba keratitis.

AB - The feasibility of inducing protective immunity to Acanthamoeba keratitis was tested in a pig model. Experiments were designed to determine if ocular infection with Acanthamoeba trophozoites would elicit protection against reinfection. Additional experiments examined whether injection of parasite antigens either intramuscularly, subconjunctivally, or by both routes would induce immunity. Therefore, four groups of animals were examined: (a) pigs that had resolved a primary corneal infection with Acanthamoeba; (b) pigs immunized intramuscularly; (c) pigs immunized subconjunctivally; and (d) pigs immunized intramuscularly and subconjunctivally. Animals were subsequently challenged with parasite-laden soft contact lenses and observed clinically for the appearance of Acanthamoeba keratitis. Acanthamoeba-specific serum antibody titers and blastogenic responses of peripheral blood lymphocytes were determined weekly. The results indicated that intramuscular injection of Acanthamoeba antigens failed to protect against ocular infection even though hosts developed high liters of IgG antibodies and displayed lymphocyte blastogenic responses to parasite antigens. Ocular infection alone failed to stimulate immunity in any of the animals. By contrast, 50% of the hosts immunized subconjunctivally were protected against corneal disease, and 100% of the animals immunized by a combination of intramuscular and sub- conjunctival administration of parasite antigens were completely protected against two separate ocular challenges with infectious parasites. Protection did not correlate with either IgG antibody titers or blastogenic potentials of peripheral blood lymphocytes. Interestingly, ocular infection alone failed to stimulate immunity to subsequent ocular challenge with infectious parasites. Thus, administration of parasite antigen via the subconjunctival route can protect against Acanthamoeba keratitis.

UR - http://www.scopus.com/inward/record.url?scp=0028898468&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028898468&partnerID=8YFLogxK

M3 - Article

VL - 14

SP - 180

EP - 186

JO - Cornea

JF - Cornea

SN - 0277-3740

IS - 2

ER -