Successive generation of antigen-presenting, dendritic cell lines from murine epidermis

S. Xu, K. Ariizumi, G. Caceres-Dittmar, D. Edelbaum, K. Hashimoto, P. R. Bergstresser, A. Takashima

Research output: Contribution to journalArticle

134 Scopus citations

Abstract

Dendritic cells are specialized APCs that exhibit an extraordinary capacity to activate naive T cells. Langerhans cells (LC), as epithelial tissue-specific members of this family, play key roles in the induction of T cell-mediated immunity against environmental, infectious, and tumor- associated Ags in skin. A major limitation in studying the biology of dendritic cells or LC has been the absence of stable, long-term cell lines. To overcome this limitation, we have established a series of APC lines (XS series) from newborn BALB/c mouse epidermis. XS lines, which have grown for more than 12 mo in culture, exhibit high similarity to LC freshly procured from skin in terms of: a) tissue of derivation (epidermis), b) phenotype (la(low)/CD45+/E-cadherin+/B7-1-), c) shape (elongated dendrites), and d) Ag-presenting profile (modest ability to activate naive, allogeneic T cells and remarkable ability to present a protein Ag to primed CD4+ T cells). The availability of XS lines as well as the methodologies used for their growth enhance our capability of studying the biology of LC at biochemical and molecular levels.

Original languageEnglish (US)
Pages (from-to)2697-2705
Number of pages9
JournalJournal of Immunology
Volume154
Issue number6
StatePublished - Jan 1 1995

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Successive generation of antigen-presenting, dendritic cell lines from murine epidermis'. Together they form a unique fingerprint.

  • Cite this

    Xu, S., Ariizumi, K., Caceres-Dittmar, G., Edelbaum, D., Hashimoto, K., Bergstresser, P. R., & Takashima, A. (1995). Successive generation of antigen-presenting, dendritic cell lines from murine epidermis. Journal of Immunology, 154(6), 2697-2705.