Successive generation of antigen-presenting, dendritic cell lines from murine epidermis

S. Xu, K. Ariizumi, G. Caceres-Dittmar, D. Edelbaum, K. Hashimoto, P. R. Bergstresser, A. Takashima

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Abstract

Dendritic cells are specialized APCs that exhibit an extraordinary capacity to activate naive T cells. Langerhans cells (LC), as epithelial tissue-specific members of this family, play key roles in the induction of T cell-mediated immunity against environmental, infectious, and tumor- associated Ags in skin. A major limitation in studying the biology of dendritic cells or LC has been the absence of stable, long-term cell lines. To overcome this limitation, we have established a series of APC lines (XS series) from newborn BALB/c mouse epidermis. XS lines, which have grown for more than 12 mo in culture, exhibit high similarity to LC freshly procured from skin in terms of: a) tissue of derivation (epidermis), b) phenotype (la(low)/CD45+/E-cadherin+/B7-1-), c) shape (elongated dendrites), and d) Ag-presenting profile (modest ability to activate naive, allogeneic T cells and remarkable ability to present a protein Ag to primed CD4+ T cells). The availability of XS lines as well as the methodologies used for their growth enhance our capability of studying the biology of LC at biochemical and molecular levels.

Original languageEnglish (US)
Pages (from-to)2697-2705
Number of pages9
JournalJournal of Immunology
Volume154
Issue number6
StatePublished - 1995

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Langerhans Cells
Antigen-Presenting Cells
Epidermis
Dendritic Cells
T-Lymphocytes
Cell Line
Aptitude
Cadherins
Skin
Dendrites
Cellular Immunity
Epithelium
Phenotype
Growth
Neoplasms
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Xu, S., Ariizumi, K., Caceres-Dittmar, G., Edelbaum, D., Hashimoto, K., Bergstresser, P. R., & Takashima, A. (1995). Successive generation of antigen-presenting, dendritic cell lines from murine epidermis. Journal of Immunology, 154(6), 2697-2705.

Successive generation of antigen-presenting, dendritic cell lines from murine epidermis. / Xu, S.; Ariizumi, K.; Caceres-Dittmar, G.; Edelbaum, D.; Hashimoto, K.; Bergstresser, P. R.; Takashima, A.

In: Journal of Immunology, Vol. 154, No. 6, 1995, p. 2697-2705.

Research output: Contribution to journalArticle

Xu, S, Ariizumi, K, Caceres-Dittmar, G, Edelbaum, D, Hashimoto, K, Bergstresser, PR & Takashima, A 1995, 'Successive generation of antigen-presenting, dendritic cell lines from murine epidermis', Journal of Immunology, vol. 154, no. 6, pp. 2697-2705.
Xu S, Ariizumi K, Caceres-Dittmar G, Edelbaum D, Hashimoto K, Bergstresser PR et al. Successive generation of antigen-presenting, dendritic cell lines from murine epidermis. Journal of Immunology. 1995;154(6):2697-2705.
Xu, S. ; Ariizumi, K. ; Caceres-Dittmar, G. ; Edelbaum, D. ; Hashimoto, K. ; Bergstresser, P. R. ; Takashima, A. / Successive generation of antigen-presenting, dendritic cell lines from murine epidermis. In: Journal of Immunology. 1995 ; Vol. 154, No. 6. pp. 2697-2705.
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