Sucralose activates an ERK1/2–ribosomal protein S6 signaling axis

Marcy L. Guerra, Michael A. Kalwat, Kathleen McGlynn, Melanie H. Cobb

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The sweetener sucralose can signal through its GPCR receptor to induce insulin secretion from pancreatic β cells, but the downstream signaling pathways involved are not well-understood. Here we measure responses to sucralose, glucagon-like peptide 1, and amino acids in MIN6 β cells. Our data suggest a signaling axis, whereby sucralose induces calcium and cAMP, activation of ERK1/2, and site-specific phosphorylation of ribosomal protein S6. Interestingly, sucralose acted independently of mTORC1 or ribosomal S6 kinase (RSK). These results suggest that sweeteners like sucralose can influence β-cell responses to secretagogues like glucose through metabolic as well as GPCR-mediated pathways. Future investigation of novel sweet taste receptor signaling pathways in β cells will have implications for diabetes and other emergent fields involving these receptors.

Original languageEnglish (US)
Pages (from-to)174-186
Number of pages13
JournalFEBS Open Bio
Volume7
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

trichlorosucrose
S 6
Sweetening Agents
Proteins
Ribosomal Protein S6
Ribosomal Protein S6 Kinases
Phosphorylation
Glucagon-Like Peptide 1
Medical problems
Chemical activation
Insulin
Calcium
Amino Acids
Glucose

Keywords

  • insulin secretion
  • pancreatic islet beta cells
  • sucralose
  • sweet taste receptor

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Sucralose activates an ERK1/2–ribosomal protein S6 signaling axis. / Guerra, Marcy L.; Kalwat, Michael A.; McGlynn, Kathleen; Cobb, Melanie H.

In: FEBS Open Bio, Vol. 7, No. 2, 01.02.2017, p. 174-186.

Research output: Contribution to journalArticle

Guerra, Marcy L. ; Kalwat, Michael A. ; McGlynn, Kathleen ; Cobb, Melanie H. / Sucralose activates an ERK1/2–ribosomal protein S6 signaling axis. In: FEBS Open Bio. 2017 ; Vol. 7, No. 2. pp. 174-186.
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