Suicidal membrane repair regulates phosphatidylserine externalization during apoptosis

Banafsheh Mirnikjoo, Krishnakumar Balasubramanian, Alan J. Schroit

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

One of the hallmarks of apoptosis is the redistribution of phosphatidylserine (PS) from the inner-to-outer plasma membrane (PM) leaflet, where it functions as a ligand for phagocyte recognition and the suppression of inflammatory responses. The mechanism by which apoptotic cells externalize PS has been assumed to involve "scramblases" that randomize phospholipids across the PM bilayer. These putative activities, however, have not been unequivocally proven to be responsible for the redistribution of lipids. Because elevated cytosolic Ca2+ is critical to this process and is also required for activation of lysosome-PM fusion during membrane repair, we hypothesized that apoptosis could activate a "pseudo"-membrane repair response that results in the fusion of lysosomes with the PM. Using a membrane-specific probe that labels endosomes and lysosomes and fluorescein-labeled annex in 5 that labels PS, we show that the appearance of PS at the cell surface during apoptosis is dependent on the fusion of lysosomes with the PM, a process that is inhibited with the lysosomotrophe, chloroquine. We demonstrate that apoptotic cells evoke a persistent pseudo-membrane repair response that likely redistributes lysosomal- derived PS to the PM outer leaflet that leads to membrane expansion and the formation of apoptotic blebs. Our data suggest that inhibition of lysosome-PM fusion-dependent redistribution of PS that occurs as a result of chemotherapy- and radiotherapy-induced apoptosis will prevent PS-dependent anti-inflammatory responses that preclude the development of tumor- and patient-specific immune responses.

Original languageEnglish (US)
Pages (from-to)22512-22516
Number of pages5
JournalJournal of Biological Chemistry
Volume284
Issue number34
DOIs
StatePublished - Aug 21 2009

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Phosphatidylserines
Cell membranes
Repair
Cell Membrane
Lysosomes
Apoptosis
Membranes
Fusion reactions
Membrane Fusion
Labels
Cells
Phospholipid Transfer Proteins
Chemotherapy
Endosomes
Chloroquine
Radiotherapy
Blister
Phagocytes
Fluorescein
Tumors

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

Cite this

Suicidal membrane repair regulates phosphatidylserine externalization during apoptosis. / Mirnikjoo, Banafsheh; Balasubramanian, Krishnakumar; Schroit, Alan J.

In: Journal of Biological Chemistry, Vol. 284, No. 34, 21.08.2009, p. 22512-22516.

Research output: Contribution to journalArticle

Mirnikjoo, Banafsheh ; Balasubramanian, Krishnakumar ; Schroit, Alan J. / Suicidal membrane repair regulates phosphatidylserine externalization during apoptosis. In: Journal of Biological Chemistry. 2009 ; Vol. 284, No. 34. pp. 22512-22516.
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