19F-MRS/1H-MRI dual-function probe for detection of β-galactosidase activity

Jian Xin Yu, Vikram D. Kodibagkar, Li Liu, Zhongwei Zhang, Jennifer Magnusson, Yuting Liu

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Because of the importance of lacZ gene in various applications ranging from molecular biology to clinical trials, the development of non-invasive bimodal techniques for improving precision and accuracy to assay gene expression has attracted much attention. In this paper, we propose a dual-function probe for synergistic combination of 19F-MRS/1H-MRI to simultaneously detect β-gal activity. Based on this strategy, we have designed, synthesized and characterized a series of 19F-MRS/1H-MRI reporters, and demonstrated the feasibility of 1-O-(β-d-galactopyranosyl)-3-fluorocatechol MGD-3-FCAT for assessing β-gal activity in solution and in vitro with lacZ transfected tumor cells as well, by the characterization of β-gal responsive 19F-chemical shift changes Δδ F, hydrolytic kinetics, and T 1, T 2 relaxation mapping.

Original languageEnglish (US)
Pages (from-to)2132-2142
Number of pages11
JournalChemical Science
Volume4
Issue number5
DOIs
StatePublished - Apr 2 2013

ASJC Scopus subject areas

  • Chemistry(all)

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    Yu, J. X., Kodibagkar, V. D., Liu, L., Zhang, Z., Magnusson, J., & Liu, Y. (2013). 19F-MRS/1H-MRI dual-function probe for detection of β-galactosidase activity. Chemical Science, 4(5), 2132-2142. https://doi.org/10.1039/c3sc21099e