19F-MRS/1H-MRI dual-function probe for detection of β-galactosidase activity

Jian Xin Yu, Vikram D. Kodibagkar, Li Liu, Zhongwei Zhang, Jennifer Magnusson, Yuting Liu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Because of the importance of lacZ gene in various applications ranging from molecular biology to clinical trials, the development of non-invasive bimodal techniques for improving precision and accuracy to assay gene expression has attracted much attention. In this paper, we propose a dual-function probe for synergistic combination of 19F-MRS/1H-MRI to simultaneously detect β-gal activity. Based on this strategy, we have designed, synthesized and characterized a series of 19F-MRS/1H-MRI reporters, and demonstrated the feasibility of 1-O-(β-d-galactopyranosyl)-3-fluorocatechol MGD-3-FCAT for assessing β-gal activity in solution and in vitro with lacZ transfected tumor cells as well, by the characterization of β-gal responsive 19F-chemical shift changes Δδ F, hydrolytic kinetics, and T 1, T 2 relaxation mapping.

Original languageEnglish (US)
Pages (from-to)2132-2142
Number of pages11
JournalChemical Science
Volume4
Issue number5
DOIs
StatePublished - Apr 2 2013

Fingerprint

Galactosidases
Magnetic resonance imaging
Molecular biology
Chemical shift
Gene expression
Tumors
Assays
Genes
Cells
Kinetics

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Yu, J. X., Kodibagkar, V. D., Liu, L., Zhang, Z., Magnusson, J., & Liu, Y. (2013). 19F-MRS/1H-MRI dual-function probe for detection of β-galactosidase activity. Chemical Science, 4(5), 2132-2142. https://doi.org/10.1039/c3sc21099e

19F-MRS/1H-MRI dual-function probe for detection of β-galactosidase activity. / Yu, Jian Xin; Kodibagkar, Vikram D.; Liu, Li; Zhang, Zhongwei; Magnusson, Jennifer; Liu, Yuting.

In: Chemical Science, Vol. 4, No. 5, 02.04.2013, p. 2132-2142.

Research output: Contribution to journalArticle

Yu, JX, Kodibagkar, VD, Liu, L, Zhang, Z, Magnusson, J & Liu, Y 2013, '19F-MRS/1H-MRI dual-function probe for detection of β-galactosidase activity', Chemical Science, vol. 4, no. 5, pp. 2132-2142. https://doi.org/10.1039/c3sc21099e
Yu, Jian Xin ; Kodibagkar, Vikram D. ; Liu, Li ; Zhang, Zhongwei ; Magnusson, Jennifer ; Liu, Yuting. / 19F-MRS/1H-MRI dual-function probe for detection of β-galactosidase activity. In: Chemical Science. 2013 ; Vol. 4, No. 5. pp. 2132-2142.
@article{166061e58f484e9295955a142310b617,
title = "19F-MRS/1H-MRI dual-function probe for detection of β-galactosidase activity",
abstract = "Because of the importance of lacZ gene in various applications ranging from molecular biology to clinical trials, the development of non-invasive bimodal techniques for improving precision and accuracy to assay gene expression has attracted much attention. In this paper, we propose a dual-function probe for synergistic combination of 19F-MRS/1H-MRI to simultaneously detect β-gal activity. Based on this strategy, we have designed, synthesized and characterized a series of 19F-MRS/1H-MRI reporters, and demonstrated the feasibility of 1-O-(β-d-galactopyranosyl)-3-fluorocatechol MGD-3-FCAT for assessing β-gal activity in solution and in vitro with lacZ transfected tumor cells as well, by the characterization of β-gal responsive 19F-chemical shift changes Δδ F, hydrolytic kinetics, and T 1, T 2 relaxation mapping.",
author = "Yu, {Jian Xin} and Kodibagkar, {Vikram D.} and Li Liu and Zhongwei Zhang and Jennifer Magnusson and Yuting Liu",
year = "2013",
month = "4",
day = "2",
doi = "10.1039/c3sc21099e",
language = "English (US)",
volume = "4",
pages = "2132--2142",
journal = "Chemical Science",
issn = "2041-6520",
publisher = "Royal Society of Chemistry",
number = "5",

}

TY - JOUR

T1 - 19F-MRS/1H-MRI dual-function probe for detection of β-galactosidase activity

AU - Yu, Jian Xin

AU - Kodibagkar, Vikram D.

AU - Liu, Li

AU - Zhang, Zhongwei

AU - Magnusson, Jennifer

AU - Liu, Yuting

PY - 2013/4/2

Y1 - 2013/4/2

N2 - Because of the importance of lacZ gene in various applications ranging from molecular biology to clinical trials, the development of non-invasive bimodal techniques for improving precision and accuracy to assay gene expression has attracted much attention. In this paper, we propose a dual-function probe for synergistic combination of 19F-MRS/1H-MRI to simultaneously detect β-gal activity. Based on this strategy, we have designed, synthesized and characterized a series of 19F-MRS/1H-MRI reporters, and demonstrated the feasibility of 1-O-(β-d-galactopyranosyl)-3-fluorocatechol MGD-3-FCAT for assessing β-gal activity in solution and in vitro with lacZ transfected tumor cells as well, by the characterization of β-gal responsive 19F-chemical shift changes Δδ F, hydrolytic kinetics, and T 1, T 2 relaxation mapping.

AB - Because of the importance of lacZ gene in various applications ranging from molecular biology to clinical trials, the development of non-invasive bimodal techniques for improving precision and accuracy to assay gene expression has attracted much attention. In this paper, we propose a dual-function probe for synergistic combination of 19F-MRS/1H-MRI to simultaneously detect β-gal activity. Based on this strategy, we have designed, synthesized and characterized a series of 19F-MRS/1H-MRI reporters, and demonstrated the feasibility of 1-O-(β-d-galactopyranosyl)-3-fluorocatechol MGD-3-FCAT for assessing β-gal activity in solution and in vitro with lacZ transfected tumor cells as well, by the characterization of β-gal responsive 19F-chemical shift changes Δδ F, hydrolytic kinetics, and T 1, T 2 relaxation mapping.

UR - http://www.scopus.com/inward/record.url?scp=85035238912&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85035238912&partnerID=8YFLogxK

U2 - 10.1039/c3sc21099e

DO - 10.1039/c3sc21099e

M3 - Article

AN - SCOPUS:85035238912

VL - 4

SP - 2132

EP - 2142

JO - Chemical Science

JF - Chemical Science

SN - 2041-6520

IS - 5

ER -