1H MRS characterization of neurochemical profiles in orthotopic mouse models of human brain tumors

Keith M. Hulsey, Tomoyuki Mashimo, Abhishek Banerjee, Todd C. Soesbe, Jeffrey S. Spence, Vamsidhara Vemireddy, Elizabeth A. Maher, Robert M. Bachoo, Changho Choi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Glioblastoma (GBM), the most common primary brain tumor, is resistant to currently available treatments. The development of mouse models of human GBM has provided a tool for studying mechanisms involved in tumor initiation and growth as well as a platform for preclinical investigation of new drugs. In this study we used 1H MR spectroscopy to study the neurochemical profile of a human orthotopic tumor (HOT) mouse model of human GBM. The goal of this study was to evaluate differences in metabolite concentrations in the GBM HOT mice when compared with normal mouse brain in order to determine if MRS could reliably differentiate tumor from normal brain. A TE =19ms PRESS sequence at 9.4T was used for measuring metabolite levels in 12 GBM mice and 8 healthy mice. Levels for 12 metabolites and for lipids/macromolecules at 0.9ppm and at 1.3ppm were reliably detected in all mouse spectra. The tumors had significantly lower concentrations of total creatine, GABA, glutamate, total N-acetylaspartate, aspartate, lipids/macromolecules at 0.9ppm, and lipids/macromolecules at 1.3ppm than did the brains of normal mice. The concentrations of glycine and lactate, however, were significantly higher in tumors than in normal brain.

Original languageEnglish (US)
Pages (from-to)108-115
Number of pages8
JournalNMR in Biomedicine
Volume28
Issue number1
DOIs
StatePublished - Jan 1 2015

Fingerprint

Brain Neoplasms
Tumors
Brain
Glioblastoma
Metabolites
Macromolecules
Lipids
Neoplasms
Creatine
Proton Magnetic Resonance Spectroscopy
Aspartic Acid
Glycine
gamma-Aminobutyric Acid
Glutamic Acid
Lactic Acid
Spectroscopy
Magnetic Resonance Spectroscopy
Pharmaceutical Preparations
Growth

Keywords

  • 9.4T
  • H MRS
  • GBM
  • Human orthotopic tumor (HOT) mouse model
  • Neurochemical profile
  • PRESS

ASJC Scopus subject areas

  • Spectroscopy
  • Molecular Medicine
  • Radiology Nuclear Medicine and imaging

Cite this

1H MRS characterization of neurochemical profiles in orthotopic mouse models of human brain tumors. / Hulsey, Keith M.; Mashimo, Tomoyuki; Banerjee, Abhishek; Soesbe, Todd C.; Spence, Jeffrey S.; Vemireddy, Vamsidhara; Maher, Elizabeth A.; Bachoo, Robert M.; Choi, Changho.

In: NMR in Biomedicine, Vol. 28, No. 1, 01.01.2015, p. 108-115.

Research output: Contribution to journalArticle

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