[3H]neurotensin receptor densities in human postmortem brain tissue obtained from normal and schizophrenic persons. An autoradiographic study

R. A. Lahti, E. V. Cochrane, R. C. Roberts, R. R. Conley, C. A. Tamminga

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

[3H]Neurotensin binding and autoradiographic techniques were used to determine the distribution and density of neurotensin receptors in normal and schizophrenic postmortem brain tissue. Coronal hemi-brain blocks of tissue were cut at the level of the caudate and hippocampus from frozen brain tissue obtained from normal individuals with no known psychiatric or neurologic illnesses and from schizophrenic subjects off- or on-antipsychotic drugs at the time of death. Each hemi-block was further divided, sectioned, thaw mounted on to slides, incubated with [3H]neurotensin and apposed to film. Digitized images were analyzed for binding densities. Areas of intense binding include the substantia nigra, the entorhinal cortex, superficial layers of the cingulate, middle frontal, and insular cortices; and with moderate binding in nucleus accumbens, and caudate. Schizophrenic patients off- (3 months or more) or on-antipsychotic drugs at the time of death were tested; all patients showed a reduced level of neurotensin receptors in the caudate (68% of normals), cingulate (34%) and prefrontal cortices (25%).

Original languageEnglish (US)
Pages (from-to)507-516
Number of pages10
JournalJournal of Neural Transmission
Volume105
Issue number4-5
DOIs
Publication statusPublished - 1998

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Keywords

  • Autoradiography
  • Human
  • Neurotensin
  • Postmortem
  • Receptor
  • Schizophrenia

ASJC Scopus subject areas

  • Neuroscience(all)

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