Super-resolution tracking of weak fluorescent markers in 3D: Application in dissecting the mechanics of chromosome segregation using fluorescence imaging and molecular genetics in yeast

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

We present a framework for the tracking of fluorescent tags in 4D microscopy (3D + time). It includes algorithms to separate tag signals under strong interference, allowing us to break the limits of optical resolution in light microscopy by a factor 3. We apply the package to read out chromosome dynamics in a functional, cell-based screen for proteins regulating cell division in yeast.

Original languageEnglish (US)
Title of host publication2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano
Pages61-64
Number of pages4
Volume1
StatePublished - 2004
Event2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano - Arlington, VA, United States
Duration: Apr 15 2004Apr 18 2004

Other

Other2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano
CountryUnited States
CityArlington, VA
Period4/15/044/18/04

Fingerprint

Chromosomes
Yeast
Optical microscopy
Microscopic examination
Mechanics
Fluorescence
Cells
Proteins
Imaging techniques
Genetics

ASJC Scopus subject areas

  • Engineering(all)

Cite this

Super-resolution tracking of weak fluorescent markers in 3D : Application in dissecting the mechanics of chromosome segregation using fluorescence imaging and molecular genetics in yeast. / Danuser, Gaudenz.

2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano. Vol. 1 2004. p. 61-64.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Danuser, G 2004, Super-resolution tracking of weak fluorescent markers in 3D: Application in dissecting the mechanics of chromosome segregation using fluorescence imaging and molecular genetics in yeast. in 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano. vol. 1, pp. 61-64, 2004 2nd IEEE International Symposium on Biomedical Imaging: Macro to Nano, Arlington, VA, United States, 4/15/04.
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