Super-resolution ultrasound imaging of the microvasculature in skeletal muscle: A new tool in diabetes research

Debabrata Ghosh, Jun Peng, Shashank R. Sirsi, Chieko Mineo, Robert Mattrey, Philip Shaul, Kenneth Hoyt

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Scopus citations


Attenuation in the skeletal muscle microvascular response to insulin plays a critical role in type 2 diabetes and obesity-induced insulin resistance. The goal of the project detailed in this paper was to evaluate the use of super-resolution ultrasound (SR-US) imaging for performing a quantitative analysis of insulin-mediated microvascular changes in skeletal muscle. An Acuson Sequoia 512 ultrasound (US) scanner equipped with a 15L8-S linear array transducer was used in a nonlinear imaging mode. C57BL/6J male mice fed standard chow and studied at age 13-16 wk comprised the lean group (N = 5), and 24-31 wk-old mice who received a high-fat diet provided the obese group (N = 5). After administration of a microbubble (MB) contrast agent, the proximal hindlimb adductor muscle of each animal was imaged (dynamic contrast-enhanced US, DCE-US) for 10 min at baseline and again at 1 h and towards the end of a 2 h hyperinsulinemic-euglycemic clamp. The SR-US image processing technique was developed using custom Matlab software. After collecting a stack of DCE-US images and applying a singular value decomposition (SVD)-based spatiotemporal filter, individual MBs were localized and subsequently a SR-US image was generated by mapping the cumulative MB localizations. The insulin-induced skeletal muscle microvascular recruitment resulting from the clamp procedure was evaluated for the lean and obese animals. Lean animals exhibited a considerably greater increase in microvascular recruitment in skeletal muscle during the hyperinsulinemic-euglycemic clamp procedure compared to the obese animals. These results clearly indicate insulin-induced microvascular recruitment in normal skeletal muscle and an impaired microvascular response to insulin in older obese animals.

Original languageEnglish (US)
Title of host publication2017 IEEE International Ultrasonics Symposium, IUS 2017
PublisherIEEE Computer Society
ISBN (Electronic)9781538633830
StatePublished - Oct 31 2017
Event2017 IEEE International Ultrasonics Symposium, IUS 2017 - Washington, United States
Duration: Sep 6 2017Sep 9 2017

Publication series

NameIEEE International Ultrasonics Symposium, IUS
ISSN (Print)1948-5719
ISSN (Electronic)1948-5727


Other2017 IEEE International Ultrasonics Symposium, IUS 2017
Country/TerritoryUnited States


  • Diabetes
  • Insulin resistance
  • Microbubbles
  • Microvascular recruitment
  • Singular value decomposition
  • Super-resolution ultrasound
  • Time microbubble count curve

ASJC Scopus subject areas

  • Acoustics and Ultrasonics


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