Supported lipid bilayer array to study clathrin mediated endocytosis in vitro

Hiroaki Suzuki, Thomas Pucadyil, Rajesh Ramachandran, Shoji Takeuchi, Sandra L. Schmid

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We describe our effort to realize an assay system to study clathrin-mediated endocytosis (CME) in vitro. Since CME related proteins are known to bind phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), we propose an accessible method to create an array of supported lipid bilayers (SLBs) with different lipid compositions, using a PDMS stamp and a microchannel. Formation of a uniform SLB of a complex lipid mixture containing PI(4,5)P 2 was confirmed by fluorescent imaging and fluorescence recovery after photobleaching (FRAP). Our technique should allow us to vary the concentration of PI(4,5)P2 or other membrane constituents to study their requirements. In preliminary experiments we showed that dynamin binds to the planar bilayer containing PI(4,5)P2, but without causing membrane tubulation as it does when dynamin binds liposomes.

Original languageEnglish (US)
Title of host publicationProceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007
Pages481-484
Number of pages4
StatePublished - Dec 1 2007
Event20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 - Kobe, Japan
Duration: Jan 21 2007Jan 25 2007

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
CountryJapan
CityKobe
Period1/21/071/25/07

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ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Suzuki, H., Pucadyil, T., Ramachandran, R., Takeuchi, S., & Schmid, S. L. (2007). Supported lipid bilayer array to study clathrin mediated endocytosis in vitro. In Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007 (pp. 481-484). [4433076] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).