TY - GEN
T1 - Supported lipid bilayer array to study clathrin mediated endocytosis in vitro
AU - Suzuki, Hiroaki
AU - Pucadyil, Thomas
AU - Ramachandran, Rajesh
AU - Takeuchi, Shoji
AU - Schmid, Sandra L.
PY - 2007
Y1 - 2007
N2 - We describe our effort to realize an assay system to study clathrin-mediated endocytosis (CME) in vitro. Since CME related proteins are known to bind phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), we propose an accessible method to create an array of supported lipid bilayers (SLBs) with different lipid compositions, using a PDMS stamp and a microchannel. Formation of a uniform SLB of a complex lipid mixture containing PI(4,5)P 2 was confirmed by fluorescent imaging and fluorescence recovery after photobleaching (FRAP). Our technique should allow us to vary the concentration of PI(4,5)P2 or other membrane constituents to study their requirements. In preliminary experiments we showed that dynamin binds to the planar bilayer containing PI(4,5)P2, but without causing membrane tubulation as it does when dynamin binds liposomes.
AB - We describe our effort to realize an assay system to study clathrin-mediated endocytosis (CME) in vitro. Since CME related proteins are known to bind phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), we propose an accessible method to create an array of supported lipid bilayers (SLBs) with different lipid compositions, using a PDMS stamp and a microchannel. Formation of a uniform SLB of a complex lipid mixture containing PI(4,5)P 2 was confirmed by fluorescent imaging and fluorescence recovery after photobleaching (FRAP). Our technique should allow us to vary the concentration of PI(4,5)P2 or other membrane constituents to study their requirements. In preliminary experiments we showed that dynamin binds to the planar bilayer containing PI(4,5)P2, but without causing membrane tubulation as it does when dynamin binds liposomes.
UR - http://www.scopus.com/inward/record.url?scp=52249089146&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=52249089146&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:52249089146
SN - 1424409519
SN - 9781424409518
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 481
EP - 484
BT - Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007
T2 - 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
Y2 - 21 January 2007 through 25 January 2007
ER -