We describe our effort to realize an assay system to study clathrin-mediated endocytosis (CME) in vitro. Since CME related proteins are known to bind phosphatidylinositol-4,5-bisphosphate (PI(4,5)P2), we propose an accessible method to create an array of supported lipid bilayers (SLBs) with different lipid compositions, using a PDMS stamp and a microchannel. Formation of a uniform SLB of a complex lipid mixture containing PI(4,5)P 2 was confirmed by fluorescent imaging and fluorescence recovery after photobleaching (FRAP). Our technique should allow us to vary the concentration of PI(4,5)P2 or other membrane constituents to study their requirements. In preliminary experiments we showed that dynamin binds to the planar bilayer containing PI(4,5)P2, but without causing membrane tubulation as it does when dynamin binds liposomes.