Suppression of WHITE COLLAR-independent frequency transcription by histone H3 lysine 36 methyltransferase SET-2 is necessary for clock function in Neurospora

Guangyan Sun, Zhipeng Zhou, Xiao Liu, Kexin Gai, Qingqing Liu, Joonseok Cha, Farah Naz Kaleri, Ying Wang, Qun He

Research output: Contribution to journalArticle

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Abstract

The circadian system in Neurospora is based on the transcriptional/translational feedback loops and rhythmic frequency (frq) transcription requires the WHITE COLLAR (WC) complex. Our previous paper has shown that frq could be transcribed in a WC-independent pathway in a strain lacking the histone H3K36 methyltransferase, SET-2 (su(var)3-9-enhancer-of-zestetrithorax-2) (1), but the mechanism was unclear. Here we disclose that loss of histone H3K36 methylation, due to either deletion of SET-2 or H3K36R mutation, results in arrhythmic frq transcription and loss of overt rhythmicity. Histone acetylation at frq locus increases in set-2KO mutant. Consistent with these results, loss of H3K36 methylation readers, histone deacetylase RPD-3 (reduced potassium dependence 3) or EAF-3 (essential SAS-related acetyltransferase-associated factor 3), also leads to hyperacetylation of histone at frq locus and WC-independent frq expression, suggesting that proper chromatin modification at frq locus is required for circadian clock operation. Furthermore, a mutant strain with three amino acid substitutions (histone H3 lysine 9, 14, and 18 to glutamine) was generated to mimic the strain with hyperacetylation state of histone H3. H3K9QK14QK18Q mutant exhibits the same defective clock phenotype as rpd-3KO mutant. Our results support a scenario in which H3K36 methylation is required to establish a permissive chromatin state for circadian frq transcription by maintaining proper acetylation status at frq locus.

Original languageEnglish (US)
Pages (from-to)11055-11063
Number of pages9
JournalJournal of Biological Chemistry
Volume291
Issue number21
DOIs
StatePublished - May 20 2016

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Neurospora
Methyltransferases
Transcription
Histones
Lysine
Clocks
Methylation
Acetylation
Chromatin
Circadian Clocks
Acetyltransferases
Histone Deacetylases
Periodicity
Amino Acid Substitution
Glutamine
Potassium
Substitution reactions
methylcobalamin-coenzyme M methyltransferase
Phenotype
Feedback

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology

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Suppression of WHITE COLLAR-independent frequency transcription by histone H3 lysine 36 methyltransferase SET-2 is necessary for clock function in Neurospora. / Sun, Guangyan; Zhou, Zhipeng; Liu, Xiao; Gai, Kexin; Liu, Qingqing; Cha, Joonseok; Kaleri, Farah Naz; Wang, Ying; He, Qun.

In: Journal of Biological Chemistry, Vol. 291, No. 21, 20.05.2016, p. 11055-11063.

Research output: Contribution to journalArticle

Sun, Guangyan ; Zhou, Zhipeng ; Liu, Xiao ; Gai, Kexin ; Liu, Qingqing ; Cha, Joonseok ; Kaleri, Farah Naz ; Wang, Ying ; He, Qun. / Suppression of WHITE COLLAR-independent frequency transcription by histone H3 lysine 36 methyltransferase SET-2 is necessary for clock function in Neurospora. In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 21. pp. 11055-11063.
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AU - Sun, Guangyan

AU - Zhou, Zhipeng

AU - Liu, Xiao

AU - Gai, Kexin

AU - Liu, Qingqing

AU - Cha, Joonseok

AU - Kaleri, Farah Naz

AU - Wang, Ying

AU - He, Qun

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AB - The circadian system in Neurospora is based on the transcriptional/translational feedback loops and rhythmic frequency (frq) transcription requires the WHITE COLLAR (WC) complex. Our previous paper has shown that frq could be transcribed in a WC-independent pathway in a strain lacking the histone H3K36 methyltransferase, SET-2 (su(var)3-9-enhancer-of-zestetrithorax-2) (1), but the mechanism was unclear. Here we disclose that loss of histone H3K36 methylation, due to either deletion of SET-2 or H3K36R mutation, results in arrhythmic frq transcription and loss of overt rhythmicity. Histone acetylation at frq locus increases in set-2KO mutant. Consistent with these results, loss of H3K36 methylation readers, histone deacetylase RPD-3 (reduced potassium dependence 3) or EAF-3 (essential SAS-related acetyltransferase-associated factor 3), also leads to hyperacetylation of histone at frq locus and WC-independent frq expression, suggesting that proper chromatin modification at frq locus is required for circadian clock operation. Furthermore, a mutant strain with three amino acid substitutions (histone H3 lysine 9, 14, and 18 to glutamine) was generated to mimic the strain with hyperacetylation state of histone H3. H3K9QK14QK18Q mutant exhibits the same defective clock phenotype as rpd-3KO mutant. Our results support a scenario in which H3K36 methylation is required to establish a permissive chromatin state for circadian frq transcription by maintaining proper acetylation status at frq locus.

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