Using the fluorescence-activated cell sorter (FACS), we have investigated that the Ig isotypes present on murine B cells, which can be polyclonally activated by lipopolysaccharide (LPS) in low cell density cultures. The LPS response was partly inhibited as a result of staining with anti-IgD and anti-IgM reagents, but not with anti-IgG reagents. The IgM +, IgD +, or IgG - fractionated cell populations gave both an IgM and an IgG response comparable to controls, whereas the response of the IgM -, IgD - cells was 5- to 20-fold lower. IgG - cells separated 1 day after LPS stimulation could still mount an IgM and IgG response indistinguishable from controls at the peak of the response. It is concluded that IgM +, IgD +, IgG - cells constitute the major LPS-sensitive cell population in the low cell density culture system and that IgG is not a necessary cell surface isotype for precursors of IgG-secreting cells.
|Original language||English (US)|
|Number of pages||8|
|Journal||Journal of Immunology|
|Publication status||Published - 1979|
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