Surface light chain expression in primary mediastinal large B-cell lymphomas by multiparameter flow cytometry

Olga K. Weinberg, Scott J. Rodig, Olga Pozdnyakova, Li Ren, Daniel A. Arber, Robert S. Ohgami

Research output: Contribution to journalArticlepeer-review

Abstract

Objectives: Primary mediastinal large B-cell lymphoma (PMLBL) is an aggressive B-cell lymphoma typically localized to the mediastinum. To date, no study has undertaken a comprehensive analysis of this entity by multiparameter flow cytometry. Methods: Cases of PMLBL with diagnostic flow cytometry were identified from pathology databases of Boston Children's Hospital, Brigham and Women's Hospital, and Stanford Hospital. Results: Most of these patients with PMLBL were women with a median age of 30 years who had stage 1 disease that lacked bone marrow involvement. By flow cytometry, 50% of all PMLBLs showed restricted surface immunoglobulin expression. When comparing patients with PMLBL by the absence or presence of surface light chain immunoglobulins, no differences were seen in the morphologic appearance; expression of CD23, CD30, or CD10; age at presentation; or clinical stage (P > .5 for all). In addition, both groups showed similarly good survival outcomes and were alive at last follow-up (11/14 [79%]; P = .542). Conclusions: This multi-institutional study demonstrates that 50% of PMLBLs can present with clonal surface light chain expression and that PMLBL is more immunophenotypically diverse than previously described. Furthermore, our findings suggest that the absence or presence of surface light chains should not be used as criteria for diagnosis in this disease.

Original languageEnglish (US)
Pages (from-to)635-641
Number of pages7
JournalAmerican journal of clinical pathology
Volume144
Issue number4
DOIs
StatePublished - Oct 2015
Externally publishedYes

Keywords

  • Flow cytometry
  • Primary mediastinal large B-cell lymphoma
  • Surface immunoglobulin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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