Surface modification of PLGA microspheres

M. Müller, J. Vörös, G. Csúcs, E. Walter, G. Danuser, H. P. Merkle, N. D. Spencer, M. Textor

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Microspheres made of poly(lactic-co-glycolic acid) (PLGA) are biocompatible and biodegradable, rendering them a promising tool in the context of drug delivery. However, nonspecific adsorption of plasma proteins on PLGA micro- and nanospheres is a main limitation of drug targeting. Poly(L-lysine)-g-poly(ethylene glycol) (PLL-g-PEG), physisorbed on flat metal oxide surfaces, has previously been shown to suppress protein adsorption drastically. The goal of our work was to characterize the efficiency of the protein repellent character of PLL-g-PEG on PLGA microspheres and to show the feasibility of introducing functional groups on the PLGA microspheres via functionalized PLL-g-PEG. To quantify the adsorbed amount of protein, a semiquantitative method that uses confocal laser scanning microscopy (CLSM) was applied. The first part of the experiment confirms the feasibility of introducing specific functional groups on PLL-g-PEG-coated PLGA microspheres. In the second part of the experiment, PLL-g-PEG-coated PLGA microspheres show a drastic decrease of adsorbed proteins by two orders of magnitude in comparison to uncoated PLGA microspheres. Low protein-binding, functionalizable microspheres provide a fundamental basis for the design of drug delivery and biosensor systems.

Original languageEnglish (US)
Pages (from-to)55-61
Number of pages7
JournalJournal of Biomedical Materials Research - Part A
Volume66
Issue number1
StatePublished - Jul 1 2003

Fingerprint

Microspheres
Surface treatment
Acids
Polyethylene glycols
Lysine
Proteins
Drug delivery
Functional groups
Adsorption
Milk
polylactic acid-polyglycolic acid copolymer
Nanospheres
Biosensors
Oxides
Blood Proteins
Microscopic examination
Metals
Experiments
Scanning
Plasmas

Keywords

  • Functionalized PLGA microspheres
  • PEG surface coating
  • Protein resistance
  • Quantitative confocal microscopy
  • Targeted drug delivery

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Müller, M., Vörös, J., Csúcs, G., Walter, E., Danuser, G., Merkle, H. P., ... Textor, M. (2003). Surface modification of PLGA microspheres. Journal of Biomedical Materials Research - Part A, 66(1), 55-61.

Surface modification of PLGA microspheres. / Müller, M.; Vörös, J.; Csúcs, G.; Walter, E.; Danuser, G.; Merkle, H. P.; Spencer, N. D.; Textor, M.

In: Journal of Biomedical Materials Research - Part A, Vol. 66, No. 1, 01.07.2003, p. 55-61.

Research output: Contribution to journalArticle

Müller, M, Vörös, J, Csúcs, G, Walter, E, Danuser, G, Merkle, HP, Spencer, ND & Textor, M 2003, 'Surface modification of PLGA microspheres', Journal of Biomedical Materials Research - Part A, vol. 66, no. 1, pp. 55-61.
Müller M, Vörös J, Csúcs G, Walter E, Danuser G, Merkle HP et al. Surface modification of PLGA microspheres. Journal of Biomedical Materials Research - Part A. 2003 Jul 1;66(1):55-61.
Müller, M. ; Vörös, J. ; Csúcs, G. ; Walter, E. ; Danuser, G. ; Merkle, H. P. ; Spencer, N. D. ; Textor, M. / Surface modification of PLGA microspheres. In: Journal of Biomedical Materials Research - Part A. 2003 ; Vol. 66, No. 1. pp. 55-61.
@article{eb09b4e5b87445ae9a1b2c13ad23217d,
title = "Surface modification of PLGA microspheres",
abstract = "Microspheres made of poly(lactic-co-glycolic acid) (PLGA) are biocompatible and biodegradable, rendering them a promising tool in the context of drug delivery. However, nonspecific adsorption of plasma proteins on PLGA micro- and nanospheres is a main limitation of drug targeting. Poly(L-lysine)-g-poly(ethylene glycol) (PLL-g-PEG), physisorbed on flat metal oxide surfaces, has previously been shown to suppress protein adsorption drastically. The goal of our work was to characterize the efficiency of the protein repellent character of PLL-g-PEG on PLGA microspheres and to show the feasibility of introducing functional groups on the PLGA microspheres via functionalized PLL-g-PEG. To quantify the adsorbed amount of protein, a semiquantitative method that uses confocal laser scanning microscopy (CLSM) was applied. The first part of the experiment confirms the feasibility of introducing specific functional groups on PLL-g-PEG-coated PLGA microspheres. In the second part of the experiment, PLL-g-PEG-coated PLGA microspheres show a drastic decrease of adsorbed proteins by two orders of magnitude in comparison to uncoated PLGA microspheres. Low protein-binding, functionalizable microspheres provide a fundamental basis for the design of drug delivery and biosensor systems.",
keywords = "Functionalized PLGA microspheres, PEG surface coating, Protein resistance, Quantitative confocal microscopy, Targeted drug delivery",
author = "M. M{\"u}ller and J. V{\"o}r{\"o}s and G. Cs{\'u}cs and E. Walter and G. Danuser and Merkle, {H. P.} and Spencer, {N. D.} and M. Textor",
year = "2003",
month = "7",
day = "1",
language = "English (US)",
volume = "66",
pages = "55--61",
journal = "Journal of Biomedical Materials Research - Part A",
issn = "0021-9304",
publisher = "John Wiley and Sons Inc.",
number = "1",

}

TY - JOUR

T1 - Surface modification of PLGA microspheres

AU - Müller, M.

AU - Vörös, J.

AU - Csúcs, G.

AU - Walter, E.

AU - Danuser, G.

AU - Merkle, H. P.

AU - Spencer, N. D.

AU - Textor, M.

PY - 2003/7/1

Y1 - 2003/7/1

N2 - Microspheres made of poly(lactic-co-glycolic acid) (PLGA) are biocompatible and biodegradable, rendering them a promising tool in the context of drug delivery. However, nonspecific adsorption of plasma proteins on PLGA micro- and nanospheres is a main limitation of drug targeting. Poly(L-lysine)-g-poly(ethylene glycol) (PLL-g-PEG), physisorbed on flat metal oxide surfaces, has previously been shown to suppress protein adsorption drastically. The goal of our work was to characterize the efficiency of the protein repellent character of PLL-g-PEG on PLGA microspheres and to show the feasibility of introducing functional groups on the PLGA microspheres via functionalized PLL-g-PEG. To quantify the adsorbed amount of protein, a semiquantitative method that uses confocal laser scanning microscopy (CLSM) was applied. The first part of the experiment confirms the feasibility of introducing specific functional groups on PLL-g-PEG-coated PLGA microspheres. In the second part of the experiment, PLL-g-PEG-coated PLGA microspheres show a drastic decrease of adsorbed proteins by two orders of magnitude in comparison to uncoated PLGA microspheres. Low protein-binding, functionalizable microspheres provide a fundamental basis for the design of drug delivery and biosensor systems.

AB - Microspheres made of poly(lactic-co-glycolic acid) (PLGA) are biocompatible and biodegradable, rendering them a promising tool in the context of drug delivery. However, nonspecific adsorption of plasma proteins on PLGA micro- and nanospheres is a main limitation of drug targeting. Poly(L-lysine)-g-poly(ethylene glycol) (PLL-g-PEG), physisorbed on flat metal oxide surfaces, has previously been shown to suppress protein adsorption drastically. The goal of our work was to characterize the efficiency of the protein repellent character of PLL-g-PEG on PLGA microspheres and to show the feasibility of introducing functional groups on the PLGA microspheres via functionalized PLL-g-PEG. To quantify the adsorbed amount of protein, a semiquantitative method that uses confocal laser scanning microscopy (CLSM) was applied. The first part of the experiment confirms the feasibility of introducing specific functional groups on PLL-g-PEG-coated PLGA microspheres. In the second part of the experiment, PLL-g-PEG-coated PLGA microspheres show a drastic decrease of adsorbed proteins by two orders of magnitude in comparison to uncoated PLGA microspheres. Low protein-binding, functionalizable microspheres provide a fundamental basis for the design of drug delivery and biosensor systems.

KW - Functionalized PLGA microspheres

KW - PEG surface coating

KW - Protein resistance

KW - Quantitative confocal microscopy

KW - Targeted drug delivery

UR - http://www.scopus.com/inward/record.url?scp=9244263935&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=9244263935&partnerID=8YFLogxK

M3 - Article

VL - 66

SP - 55

EP - 61

JO - Journal of Biomedical Materials Research - Part A

JF - Journal of Biomedical Materials Research - Part A

SN - 0021-9304

IS - 1

ER -