Surface preparation strategies for improved parallelization and reproducible MALDI-TOF MS ligand binding assays

Michael J. Roth; Erica M. Maresh; Daniel A. Plymire; Junmei Zhang; John R. Corbett; Roger Robbins; Steven M. Patrie

doi:10.1021/am3024756

Surface preparation strategies for improved parallelization and reproducible MALDI-TOF MS ligand binding assays

Michael J. Roth, Erica M. Maresh, Daniel A. Plymire, Junmei Zhang, John R. Corbett, Roger Robbins, Steven M. Patrie

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Immunoassays are employed in academia and the healthcare and biotech industries for high-throughput, quantitative screens of biomolecules. We have developed monolayer-based immunoassays for MALDI-TOF MS. To improve parallelization, we adapted the workflow to photolithography-generated arrays. Our work shows Parylene-C coatings provide excellent "solvent pinning" for reagents and biofluids, enabling sensitive MS detection of immobilized components. With a unique MALDI-matrix crystallization technique we show routine interassay RSD <10% at picomolar concentrations and highlight platform compatibility for relative and label-free quantitation applications. Parylene-arrays provide high sample densities and promise screening throughputs exceeding 1000 samples/h with modern liquid-handlers and MALDI-TOF systems.

Original language	English (US)
Pages (from-to)	6-10
Number of pages	5
Journal	ACS Applied Materials and Interfaces
Volume	5
Issue number	1
DOIs	https://doi.org/10.1021/am3024756
State	Published - Jan 9 2013

Keywords

MALDI-TOF
arrays
biomarker
immunoassays
mass spectrometry
self-assembled monolayers

ASJC Scopus subject areas

Materials Science(all)

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10.1021/am3024756

Cite this

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AU - Maresh, Erica M.

AU - Plymire, Daniel A.

AU - Zhang, Junmei

AU - Corbett, John R.

AU - Robbins, Roger

AU - Patrie, Steven M.

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KW - biomarker

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KW - mass spectrometry

KW - self-assembled monolayers

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Surface preparation strategies for improved parallelization and reproducible MALDI-TOF MS ligand binding assays

Abstract

Keywords

ASJC Scopus subject areas

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