Surface preparation strategies for improved parallelization and reproducible MALDI-TOF MS ligand binding assays

Michael J. Roth, Erica M. Maresh, Daniel A. Plymire, Junmei Zhang, John R. Corbett, Roger Robbins, Steven M. Patrie

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Immunoassays are employed in academia and the healthcare and biotech industries for high-throughput, quantitative screens of biomolecules. We have developed monolayer-based immunoassays for MALDI-TOF MS. To improve parallelization, we adapted the workflow to photolithography-generated arrays. Our work shows Parylene-C coatings provide excellent "solvent pinning" for reagents and biofluids, enabling sensitive MS detection of immobilized components. With a unique MALDI-matrix crystallization technique we show routine interassay RSD <10% at picomolar concentrations and highlight platform compatibility for relative and label-free quantitation applications. Parylene-arrays provide high sample densities and promise screening throughputs exceeding 1000 samples/h with modern liquid-handlers and MALDI-TOF systems.

Original languageEnglish (US)
Pages (from-to)6-10
Number of pages5
JournalACS Applied Materials and Interfaces
Volume5
Issue number1
DOIs
StatePublished - Jan 9 2013

Keywords

  • MALDI-TOF
  • arrays
  • biomarker
  • immunoassays
  • mass spectrometry
  • self-assembled monolayers

ASJC Scopus subject areas

  • Materials Science(all)

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