Surface preparation strategies for improved parallelization and reproducible MALDI-TOF MS ligand binding assays

Michael J. Roth, Erica M. Maresh, Daniel A. Plymire, Junmei Zhang, John R. Corbett, Roger Robbins, Steven M. Patrie

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Immunoassays are employed in academia and the healthcare and biotech industries for high-throughput, quantitative screens of biomolecules. We have developed monolayer-based immunoassays for MALDI-TOF MS. To improve parallelization, we adapted the workflow to photolithography-generated arrays. Our work shows Parylene-C coatings provide excellent "solvent pinning" for reagents and biofluids, enabling sensitive MS detection of immobilized components. With a unique MALDI-matrix crystallization technique we show routine interassay RSD <10% at picomolar concentrations and highlight platform compatibility for relative and label-free quantitation applications. Parylene-arrays provide high sample densities and promise screening throughputs exceeding 1000 samples/h with modern liquid-handlers and MALDI-TOF systems.

Original languageEnglish (US)
Pages (from-to)6-10
Number of pages5
JournalACS Applied Materials and Interfaces
Volume5
Issue number1
DOIs
StatePublished - Jan 9 2013

Fingerprint

Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Assays
Ligands
Throughput
Immunoassay
Biomolecules
Photolithography
Crystallization
Labels
Monolayers
Screening
Health Care Sector
Workflow
Coatings
Liquids
Industry
parylene

Keywords

  • arrays
  • biomarker
  • immunoassays
  • MALDI-TOF
  • mass spectrometry
  • self-assembled monolayers

ASJC Scopus subject areas

  • Materials Science(all)
  • Medicine(all)

Cite this

Surface preparation strategies for improved parallelization and reproducible MALDI-TOF MS ligand binding assays. / Roth, Michael J.; Maresh, Erica M.; Plymire, Daniel A.; Zhang, Junmei; Corbett, John R.; Robbins, Roger; Patrie, Steven M.

In: ACS Applied Materials and Interfaces, Vol. 5, No. 1, 09.01.2013, p. 6-10.

Research output: Contribution to journalArticle

Roth, Michael J. ; Maresh, Erica M. ; Plymire, Daniel A. ; Zhang, Junmei ; Corbett, John R. ; Robbins, Roger ; Patrie, Steven M. / Surface preparation strategies for improved parallelization and reproducible MALDI-TOF MS ligand binding assays. In: ACS Applied Materials and Interfaces. 2013 ; Vol. 5, No. 1. pp. 6-10.
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