Surgical models for cardiac regeneration in neonatal mice

Ahmed I. Mahmoud, Enzo R. Porrello, Wataru Kimura, Eric N. Olson, Hesham A. Sadek

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Although amphibian and fish models of heart regeneration have existed for decades, a mammalian equivalent has long remained elusive. Our discovery of a brief postnatal window for heart regeneration in neonatal mice has led to the establishment of surgical models for cardiac regenerative studies in mammals for the first time. This protocol describes a 10-min surgical procedure to induce cardiac injury in 1-d-old neonatal mice. This allows for the analysis of cardiac regeneration after surgical amputation of the left ventricle (LV) (apical resection) and coronary artery occlusion (myocardial infarction (MI)). A comparative analysis of neonatal and adult responses to myocardial injury should enable identification of the key differences between regenerative and nonregenerative responses to cardiac injury. This protocol can also be adapted to the growing repertoire of genetic models available in the mouse, and it provides a valuable tool for unlocking the molecular mechanisms that guide mammalian heart regeneration during early postnatal life.

Original languageEnglish (US)
Pages (from-to)305-311
Number of pages7
JournalNature Protocols
Volume9
Issue number2
DOIs
StatePublished - Feb 2014

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Anatomic Models
Regeneration
Wounds and Injuries
Mammals
Fish
Coronary Occlusion
Genetic Models
Amphibians
Amputation
Heart Ventricles
Coronary Vessels
Fishes
Myocardial Infarction

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Surgical models for cardiac regeneration in neonatal mice. / Mahmoud, Ahmed I.; Porrello, Enzo R.; Kimura, Wataru; Olson, Eric N.; Sadek, Hesham A.

In: Nature Protocols, Vol. 9, No. 2, 02.2014, p. 305-311.

Research output: Contribution to journalArticle

Mahmoud, Ahmed I. ; Porrello, Enzo R. ; Kimura, Wataru ; Olson, Eric N. ; Sadek, Hesham A. / Surgical models for cardiac regeneration in neonatal mice. In: Nature Protocols. 2014 ; Vol. 9, No. 2. pp. 305-311.
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