Synapsin I is structurally similar to ATP-utilizing enzymes

Lothar Esser, Chyung Ru Wang, Masahiro Hosaka, Cynthia S. Smagula, Thomas C.südhof, Johann Deisenhofer

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Synapsins are abundant synaptic vesicle proteins with an essential regulatory function in the nerve terminal. We determined the crystal structure of a fragment (synC) consisting of residues 110-420 of bovine synapsin I; synC coincides with the large middle domain (C-domain), the most conserved domain of synapsins. SynC molecules are folded into compact domains and form closely associated dimers. SynC monomers are strikingly similar in structure to a family of ATP-utilizing enzymes, which includes glutathione synthetase and D-alanine:D-alanine ligase. SynC binds ATP in a Ca2+-dependent manner. The crystal structure of synC in complex with ATPγS and Ca2+ explains the preference of synC for Ca2+ over Mg2+. Our results suggest that synapsins may also be ATP-utilizing enzymes.

Original languageEnglish (US)
Pages (from-to)977-984
Number of pages8
JournalEMBO Journal
Volume17
Issue number4
DOIs
StatePublished - Feb 4 1998

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Synapsins
Adenosine Triphosphate
Enzymes
Glutathione Synthase
Crystal structure
Synaptic Vesicles
Dimers
Monomers
Molecules
Proteins

Keywords

  • ATP binding
  • Ca binding
  • Crystal structure
  • Structural similarity
  • Synaptic vesicle proteins

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Esser, L., Wang, C. R., Hosaka, M., Smagula, C. S., C.südhof, T., & Deisenhofer, J. (1998). Synapsin I is structurally similar to ATP-utilizing enzymes. EMBO Journal, 17(4), 977-984. https://doi.org/10.1093/emboj/17.4.977

Synapsin I is structurally similar to ATP-utilizing enzymes. / Esser, Lothar; Wang, Chyung Ru; Hosaka, Masahiro; Smagula, Cynthia S.; C.südhof, Thomas; Deisenhofer, Johann.

In: EMBO Journal, Vol. 17, No. 4, 04.02.1998, p. 977-984.

Research output: Contribution to journalArticle

Esser, L, Wang, CR, Hosaka, M, Smagula, CS, C.südhof, T & Deisenhofer, J 1998, 'Synapsin I is structurally similar to ATP-utilizing enzymes', EMBO Journal, vol. 17, no. 4, pp. 977-984. https://doi.org/10.1093/emboj/17.4.977
Esser L, Wang CR, Hosaka M, Smagula CS, C.südhof T, Deisenhofer J. Synapsin I is structurally similar to ATP-utilizing enzymes. EMBO Journal. 1998 Feb 4;17(4):977-984. https://doi.org/10.1093/emboj/17.4.977
Esser, Lothar ; Wang, Chyung Ru ; Hosaka, Masahiro ; Smagula, Cynthia S. ; C.südhof, Thomas ; Deisenhofer, Johann. / Synapsin I is structurally similar to ATP-utilizing enzymes. In: EMBO Journal. 1998 ; Vol. 17, No. 4. pp. 977-984.
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