Synaptic targeting of N-type calcium channels in hippocampal neurons

Anton Maximov, Ilya Bezprozvanny

Research output: Contribution to journalArticle

146 Scopus citations

Abstract

N-type calcium (Ca2+) channels play a critical role in synaptic function, but the mechanisms responsible for their targeting in neurons are poorly understood. N-type channels are formed by an α1B (Cav2.2) pore-forming subunit associated with β and α2δ auxiliary subunits. By expressing epitope-tagged recombinant α1B subunits in rat hippocampal neuronal cultures, we demonstrate here that synaptic targeting of N-type channels depends on neuronal contacts and synapse formation. We also establish that the C-terminal 163 aa (2177-2339) of the α1B-1 (Cav2.2a) splice variant contain sequences that are both necessary and sufficient for synaptic targeting. By site-directed mutagenesis, we demonstrate that postsynaptic density-95/discs large/zona occludens-1 and Src homology 3 domain-binding motifs located within this region of the α1B subunit (Maximov et al., 1999) act as synergistic synaptic targeting signals. We also show that the recombinant modular adaptor proteins Mint1 and CASK colocalize with N-type channels in synapses. We found that the α1B-2 (Cav2.2b) splice variant is restricted to soma and dendrites and postulated that somatodendritic and axonal/presynaptic isoforms of N-type channels are generated via alternative splicing of α1B C termini. These data lead us to propose that during synaptogenesis, the α1B-1 (Cav2.2a) splice variant of the N-type Ca2+ channel pore-forming subunit is recruited to presynaptic locations by means of interactions with modular adaptor proteins Mint1 and CASK. Our results provide a novel insight into the molecular mechanisms responsible for targeting of Ca2+ channels and other synaptic proteins in neurons.

Original languageEnglish (US)
Pages (from-to)6939-6952
Number of pages14
JournalJournal of Neuroscience
Volume22
Issue number16
StatePublished - Aug 15 2002

Keywords

  • Axons
  • Calcium channels
  • PDZ domains
  • Polarized cells
  • Protein targeting
  • Synapse

ASJC Scopus subject areas

  • Neuroscience(all)

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