Synaptobrevin is essential for fast synaptic-vesicle endocytosis

Ferenc Deák, Susanne Schoch, Xinran Liu, Thomas C. Südhof, Ege T. Kavalali

Research output: Contribution to journalArticlepeer-review

176 Scopus citations

Abstract

Synaptobrevin-2 (VAMP-2), the major SNARE protein of synaptic vesicles, is required for fast calcium-triggered synaptic-vesicle exocytosis. Here we show that synaptobrevin-2 is also essential for fast synaptic-vesicle endocytosis. We demonstrate that after depletion of the readily releasable vesicle pool, replenishment of the pool is delayed by knockout of synaptobrevin. This delay was not from a loss of vesicles, because the total number of pre-synaptic vesicles, docked vesicles and actively recycling vesicles was unaffected. However, altered shape and size of the vesicles in synaptobrevin-deficient synapses suggests a defect in endocytosis. Consistent with such a defect, the stimulus-dependent endocytosis of horseradish peroxidase and fluorescent FM1-43 were delayed, indicating that fast vesicle endocytosis may normally be nucleated by a SNARE-dependent coat. Thus, synaptobrevin is essential for two fast synapse-specific membrane trafficking reactions: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid reuse of synaptic vesicles.

Original languageEnglish (US)
Pages (from-to)1102-1108
Number of pages7
JournalNature cell biology
Volume6
Issue number11
DOIs
StatePublished - Nov 1 2004

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Synaptobrevin is essential for fast synaptic-vesicle endocytosis'. Together they form a unique fingerprint.

Cite this