Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating

Patrik Verstreken, Tong Wey Koh, Karen L. Schulze, R. Grace Zhai, P. Robin Hiesinger, Yi Zhou, Sunil Q. Mehta, Yu Cao, Jack Roos, Hugo J. Bellen

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Abstract

We describe the isolation and characterization of Drosophila synaptojanin (synj) mutants. synj encodes a phosphatidylinositol phosphatase involved in clathrin-mediated endocytosis. We show that Synj is specifically localized to presynaptic terminals and is associated with synaptic vesicles. The electrophysiological and ultrastructural defects observed in synj mutants are strikingly similar to those found in endophilin mutants, and Synj and Endo colocalize and interact biochemically. Moreover, synj; endo double mutant synaptic terminals exhibit properties that are very similar to terminals of each single mutant, and overexpression of Endophilin can partially rescue the functional defects in partial loss-of-function synj mutants. Interestingly, Synj is mislocalized and destabilized at synapses devoid of Endophilin, suggesting that Endophilin recruits and stabilizes Synj on newly formed vesicles to promote vesicle uncoating. Our data also provide further evidence that kiss-and-run is able to maintain neurotransmitter release when synapses are not extensively challenged.

Original languageEnglish (US)
Pages (from-to)733-748
Number of pages16
JournalNeuron
Volume40
Issue number4
DOIs
StatePublished - Nov 13 2003

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ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Verstreken, P., Koh, T. W., Schulze, K. L., Zhai, R. G., Hiesinger, P. R., Zhou, Y., Mehta, S. Q., Cao, Y., Roos, J., & Bellen, H. J. (2003). Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating. Neuron, 40(4), 733-748. https://doi.org/10.1016/S0896-6273(03)00644-5