Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating

Patrik Verstreken, Tong Wey Koh, Karen L. Schulze, R. Grace Zhai, P. Robin Hiesinger, Yi Zhou, Sunil Q. Mehta, Yu Cao, Jack Roos, Hugo J. Bellen

Research output: Contribution to journalArticle

258 Citations (Scopus)

Abstract

We describe the isolation and characterization of Drosophila synaptojanin (synj) mutants. synj encodes a phosphatidylinositol phosphatase involved in clathrin-mediated endocytosis. We show that Synj is specifically localized to presynaptic terminals and is associated with synaptic vesicles. The electrophysiological and ultrastructural defects observed in synj mutants are strikingly similar to those found in endophilin mutants, and Synj and Endo colocalize and interact biochemically. Moreover, synj; endo double mutant synaptic terminals exhibit properties that are very similar to terminals of each single mutant, and overexpression of Endophilin can partially rescue the functional defects in partial loss-of-function synj mutants. Interestingly, Synj is mislocalized and destabilized at synapses devoid of Endophilin, suggesting that Endophilin recruits and stabilizes Synj on newly formed vesicles to promote vesicle uncoating. Our data also provide further evidence that kiss-and-run is able to maintain neurotransmitter release when synapses are not extensively challenged.

Original languageEnglish (US)
Pages (from-to)733-748
Number of pages16
JournalNeuron
Volume40
Issue number4
DOIs
StatePublished - Nov 13 2003

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Synaptic Vesicles
Presynaptic Terminals
Synapses
Clathrin
Phosphatidylinositols
Endocytosis
Phosphoric Monoester Hydrolases
Drosophila
Neurotransmitter Agents
synaptojanin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Verstreken, P., Koh, T. W., Schulze, K. L., Zhai, R. G., Hiesinger, P. R., Zhou, Y., ... Bellen, H. J. (2003). Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating. Neuron, 40(4), 733-748. https://doi.org/10.1016/S0896-6273(03)00644-5

Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating. / Verstreken, Patrik; Koh, Tong Wey; Schulze, Karen L.; Zhai, R. Grace; Hiesinger, P. Robin; Zhou, Yi; Mehta, Sunil Q.; Cao, Yu; Roos, Jack; Bellen, Hugo J.

In: Neuron, Vol. 40, No. 4, 13.11.2003, p. 733-748.

Research output: Contribution to journalArticle

Verstreken, P, Koh, TW, Schulze, KL, Zhai, RG, Hiesinger, PR, Zhou, Y, Mehta, SQ, Cao, Y, Roos, J & Bellen, HJ 2003, 'Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating', Neuron, vol. 40, no. 4, pp. 733-748. https://doi.org/10.1016/S0896-6273(03)00644-5
Verstreken P, Koh TW, Schulze KL, Zhai RG, Hiesinger PR, Zhou Y et al. Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating. Neuron. 2003 Nov 13;40(4):733-748. https://doi.org/10.1016/S0896-6273(03)00644-5
Verstreken, Patrik ; Koh, Tong Wey ; Schulze, Karen L. ; Zhai, R. Grace ; Hiesinger, P. Robin ; Zhou, Yi ; Mehta, Sunil Q. ; Cao, Yu ; Roos, Jack ; Bellen, Hugo J. / Synaptojanin is recruited by endophilin to promote synaptic vesicle uncoating. In: Neuron. 2003 ; Vol. 40, No. 4. pp. 733-748.
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