@article{72bbbbdeb1a44e13844f077aecafea63,
title = "Synaptotagmin I: A major Ca2+ sensor for transmitter release at a central synapse",
abstract = "Mice carrying a mutation in the synaptotagmin I gene were generated by homologous recombination. Mutant mice are phenotypically normal as heterozygotes, but die within 48 hr after birth as homozygotes. Studies of hippocampal neurons cultured from homozygous mutant mice reveal that synaptic transmission is severely impaired. The synchronous, fast component of Ca2+-dependent neurotransmitter release is decreased, whereas asynchronous release processes, including spontaneous synaptic activity (miniature excitatory postsynaptic current frequency) and release triggered by hypertonic solution or α-latrotoxin, are unaffected. Our findings demonstrate that synaptotagmin I function is required for Ca2+-triggering of synchronous neurotransmitter release, but is not essential for asynchronous or Ca2+-independent release. We propose that synaptotagmin I is the major low affinity Ca2+ sensor mediating Ca2+ regulation of synchronous neurotransmitter release in hippocampal neurons.",
author = "Martin Geppert and Yukiko Goda and Hammer, {Robert E} and Cai Li and Rosahl, {Thomas W.} and Stevens, {Charles F.} and S{\"u}dhof, {Thomas C.}",
note = "Funding Information: The first two authors contributed equally to this work. Correspondence should be addressed to T. C. S. We are indebted to Richard Jacobs for preparing the electron micrographs of wild-type and mutant synapses in hippocampal cultures. We would like tothank Drs. P. Soriano, Ft. Jahn, J. Herz, N. Brose, and A. Bradley for their gifts of reagents; Drs. J. L. Goldstein and M. S. Brown for advice; and Ms. I. Leznicki, L. Lundquist, and A. Hopkins for excellent technical assistance. This study was supported by the Howard Hughes Medical Institute (Ft. E. H., T. C. S., and C. F. S.), grants from the W. M. Keck Foundation (R. E. H. and T. C. S.), the Perot Family Foundation (l. C. S.), and National Institutes of Health grant ROI NS 12961-17 (C. F. S.). Y. G. is supported by a Young Investigator Award from the National Alliance for Research on Schizophrenia and Depression. M. G. and T. R. were partly supported by the Deutsche Forschungsgemeinschaft.",
year = "1994",
month = nov,
day = "18",
doi = "10.1016/0092-8674(94)90556-8",
language = "English (US)",
volume = "79",
pages = "717--727",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "4",
}