TY - JOUR
T1 - Synaptotagmin regulation of coated pit assembly
AU - Von Poser, Christine
AU - Zhang, Jimmy Z.
AU - Mineo, Chieko
AU - Ding, Wei
AU - Ying, Yunshu
AU - Südhof, Thomas C.
AU - Anderson, Richard G W
PY - 2000/10/6
Y1 - 2000/10/6
N2 - Synaptotagmins bind clathrin AP-2 with high affinity via their second C2 domain, which indicates they are involved in coated pit function. We now report that expression of synaptotagmins lacking either the second C2 domain or the entire cytoplasmic region potently inhibit endocytosis. Inhibition was dependent on two intramembrane cysteine residues that were found to be essential for synaptotagmin oligomerization. Cells expressing the wild-type, but not the mutant, truncated synaptotagmin fragment had a reduced number of clathrin-coated pits. These results suggest that the formation of synaptotagmin multimers is an important step in the regulation of coated pit assembly.
AB - Synaptotagmins bind clathrin AP-2 with high affinity via their second C2 domain, which indicates they are involved in coated pit function. We now report that expression of synaptotagmins lacking either the second C2 domain or the entire cytoplasmic region potently inhibit endocytosis. Inhibition was dependent on two intramembrane cysteine residues that were found to be essential for synaptotagmin oligomerization. Cells expressing the wild-type, but not the mutant, truncated synaptotagmin fragment had a reduced number of clathrin-coated pits. These results suggest that the formation of synaptotagmin multimers is an important step in the regulation of coated pit assembly.
UR - http://www.scopus.com/inward/record.url?scp=0034613154&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0034613154&partnerID=8YFLogxK
U2 - 10.1074/jbc.M005559200
DO - 10.1074/jbc.M005559200
M3 - Article
C2 - 10906143
AN - SCOPUS:0034613154
SN - 0021-9258
VL - 275
SP - 30916
EP - 30924
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 40
ER -