Synthesis, analysis, purification, and intracellular delivery of peptide nucleic acids

Dwaine A. Braasch, David R. Corey

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

Peptide nucleic acids (PNAs) are nonionic DNA mimics. Their novel chemical properties may facilitate the development of selective and potent antisense and antigene strategies for regulating intracellular processes. Described herein are procedures for the synthesis, purification, handling, and characterization of PNAs. A simple protocol for the lipid-mediated introduction of PNAs into in vitro cultures of mammalian cells is provided.

Original languageEnglish (US)
Pages (from-to)97-107
Number of pages11
JournalMethods
Volume23
Issue number2
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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