Synthesis, analysis, purification, and intracellular delivery of peptide nucleic acids

Dwaine A. Braasch; David R. Corey

doi:10.1006/meth.2000.1111

Synthesis, analysis, purification, and intracellular delivery of peptide nucleic acids

Dwaine A. Braasch, David R. Corey

Research output: Contribution to journal › Article › peer-review

56 Scopus citations

Abstract

Peptide nucleic acids (PNAs) are nonionic DNA mimics. Their novel chemical properties may facilitate the development of selective and potent antisense and antigene strategies for regulating intracellular processes. Described herein are procedures for the synthesis, purification, handling, and characterization of PNAs. A simple protocol for the lipid-mediated introduction of PNAs into in vitro cultures of mammalian cells is provided.

Original language	English (US)
Pages (from-to)	97-107
Number of pages	11
Journal	Methods
Volume	23
Issue number	2
DOIs	https://doi.org/10.1006/meth.2000.1111
State	Published - 2001

ASJC Scopus subject areas

Molecular Biology
General Biochemistry, Genetics and Molecular Biology

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10.1006/meth.2000.1111

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title = "Synthesis, analysis, purification, and intracellular delivery of peptide nucleic acids",

abstract = "Peptide nucleic acids (PNAs) are nonionic DNA mimics. Their novel chemical properties may facilitate the development of selective and potent antisense and antigene strategies for regulating intracellular processes. Described herein are procedures for the synthesis, purification, handling, and characterization of PNAs. A simple protocol for the lipid-mediated introduction of PNAs into in vitro cultures of mammalian cells is provided.",

author = "Braasch, {Dwaine A.} and Corey, {David R.}",

note = "Funding Information: This work was supported by grants from the National Institutes of Health (CA 74908, GM 60624) and the Robert A. Welch Foundation (I±1244).",

year = "2001",

doi = "10.1006/meth.2000.1111",

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pages = "97--107",

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AU - Braasch, Dwaine A.

AU - Corey, David R.

N1 - Funding Information: This work was supported by grants from the National Institutes of Health (CA 74908, GM 60624) and the Robert A. Welch Foundation (I±1244).

PY - 2001

Y1 - 2001

N2 - Peptide nucleic acids (PNAs) are nonionic DNA mimics. Their novel chemical properties may facilitate the development of selective and potent antisense and antigene strategies for regulating intracellular processes. Described herein are procedures for the synthesis, purification, handling, and characterization of PNAs. A simple protocol for the lipid-mediated introduction of PNAs into in vitro cultures of mammalian cells is provided.

AB - Peptide nucleic acids (PNAs) are nonionic DNA mimics. Their novel chemical properties may facilitate the development of selective and potent antisense and antigene strategies for regulating intracellular processes. Described herein are procedures for the synthesis, purification, handling, and characterization of PNAs. A simple protocol for the lipid-mediated introduction of PNAs into in vitro cultures of mammalian cells is provided.

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Synthesis, analysis, purification, and intracellular delivery of peptide nucleic acids

Abstract

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