@article{fc02de84f1974e0db6aa8c0851393805,
title = "Synthesis and membrane permeability of PNA-peptide conjugates",
abstract = "Chimeric molecules consisting of peptide nucleic acid oligomers (PNAs) and peptides derived from the third helix of the homeodomain of Antennapedia are taken up by mammalian cells in culture. Uptake is independent of orientation and occurs with high efficiency, suggesting that peptide conjugates are a promising strategy for intracellular PNA delivery.",
author = "Simmons, {Carla G.} and Pitts, {Anne E.} and Mayfield, {Lynn D.} and Shay, {Jerry W.} and Corey, {David R.}",
note = "Funding Information: Analysis by flow cytometry. Adherent populations of DUI45 cells were treated with 1 laM rhodamine labeled conjugate 1, the analogous rhodamine labeled PNA, or unlabeled PNA in media for 2 h at 37 °C. Cells were extensively washed, trypsinized, and resuspended in 0.5 mL 1 X PBS. Populations were immediately analyzed on a FACStarPlus flow cytometer using LYSYS II software (Becton Dickinson, Franklin Lakes, N J) and a 575 nm broad band pass filter. Cell populations were gated to only measure the fluorescence in intact cells. Acknowledgements We would like to thank Steve Madden for peptide synthesis, Revathi Katipally for PNA synthesis, and Lauren Gollahon and Susan Hamilton for advice. This work was supported by a grant from the Robert A. Welch Foundation (I-1244). Anne Pitts was supported by NIH predoctoral training grant 5T32GM0706223. DRC is an assistant investigator with the Howard Hughes Medical Institute. References 1. Nielsen, P. E.; Egholm, M.; Berg, R. H.; Buchardt, O. Science 1991, 254, 1497. 2. Egholm, M.; Buchardt, O.; Christensen, L.; Behrens, C.; Freier, S. M.; Driver, D. A.; Berg, R. H.; Kim, S. K.; Nordem, B.; Neilsen, P. E. Nature (London) 1993, 365, 566.",
year = "1997",
month = dec,
day = "2",
doi = "10.1016/S0960-894X(97)10136-6",
language = "English (US)",
volume = "7",
pages = "3001--3006",
journal = "Bioorganic and Medicinal Chemistry Letters",
issn = "0960-894X",
publisher = "Elsevier Limited",
number = "23",
}