Abstract
A new, UV-excited fluorescent Zn2+ indicator was synthesized and the spectral profile of its free and Zn2+ bound forms were studied. The fluorescent properties of this probe are due to the 7-amino-4-methylcoumarin fluorophore, which is conjugated with the tris(2-aminoethyl)amine (TREN) that functions as the zinc-chelating moiety. The compound exhibits a Zn2+ dissociation constant of 18.0 μM. The fluorescence spectra of the probe showed a clear shift in excitation wavelength maxima upon Zn2+ binding, indicating its potential use as ratiometric Zn2+ indicator.
Original language | English (US) |
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Pages (from-to) | 4193-4196 |
Number of pages | 4 |
Journal | Tetrahedron Letters |
Volume | 46 |
Issue number | 24 |
DOIs | |
State | Published - Jun 13 2005 |
ASJC Scopus subject areas
- Biochemistry
- Drug Discovery
- Organic Chemistry