Synthesis, intracellular distribution, and secretion of immunoglobulin and H 2 antigen in murine splenocytes

D. Wernet, E. S. Vitetta, J. W. Uhr, E. A. Boyse

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A/J spleen cells were labeled with [3H]leucine and at intervals thereafter were homogenized and separated into microsomes and cell sap. Ig and H 2 antigens were assayed in the cell fractions and cell supernatants using immunoprecipitation. In addition, cells labeled by enzymatic radioiodination were incubated to determine the rates of release of Ig and H 2 antigens from the surface. The results indicate that the majority of Ig and H 2 antigens remain membrane bound throughout their intracellular life. In contrast to Ig, H 2 antigens are neither secreted nor shed from the cell surface. It is suggested that Ig is a peripheral protein of the cell membrane, whereas H 2 antigens are integral ones. The release of Ig on a fragment of plasma membrane could occur at fixed cell surface areas that contain no H 2 antigens or from which they have migrated before release.

Original languageEnglish (US)
Pages (from-to)847-857
Number of pages11
JournalJournal of Experimental Medicine
Volume138
Issue number4
StatePublished - 1973

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H-2 Antigens
Immunoglobulins
Microsomes
Immunoprecipitation
Leucine
Membrane Proteins
Spleen
Cell Membrane
Membranes

ASJC Scopus subject areas

  • Immunology

Cite this

Synthesis, intracellular distribution, and secretion of immunoglobulin and H 2 antigen in murine splenocytes. / Wernet, D.; Vitetta, E. S.; Uhr, J. W.; Boyse, E. A.

In: Journal of Experimental Medicine, Vol. 138, No. 4, 1973, p. 847-857.

Research output: Contribution to journalArticle

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