Synthesis of cholesterol in the human fetus: 3-hydroxy-3-methylglutaryl coenzyme a reductase activity of liver microsomes

B. R. Carr; E. R. Simpson

doi:10.1210/jcem-53-4-810

Synthesis of cholesterol in the human fetus: 3-hydroxy-3-methylglutaryl coenzyme a reductase activity of liver microsomes

B. R. Carr, E. R. Simpson

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The specific activity of 3-hydroxy-3-methylglu-taryl coenzyme A (HMG CoA) reductase in microsome-enriched fractions prepared from normal human fetal liver tissue was assessed. The mean specific activity was 0.58 ± 0.18 nmol mev-alonate formed min⁻¹ mg⁻¹ protein. The activity of the enzyme was inhibited by preincubation of microsomes with ATP (4 m.M) and was greatly reduced when microsomes were prepared from tissue homogenized in the presence of NaF (50 HIM). It can be computed that the activity of HMG CoA reductase in human fetal liver microsomes is adequate to provide cholesterol to meet the requirements of the fetal adrenal for steroid precursor provided that cholesterol synthesized in the fetal liver appears in the plasma in the form of low density lipoprotein.

Original language	English (US)
Pages (from-to)	810-812
Number of pages	3
Journal	Journal of Clinical Endocrinology and Metabolism
Volume	53
Issue number	4
DOIs	https://doi.org/10.1210/jcem-53-4-810
State	Published - Oct 1981

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Biochemistry
Endocrinology
Clinical Biochemistry
Biochemistry, medical

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10.1210/jcem-53-4-810

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title = "Synthesis of cholesterol in the human fetus: 3-hydroxy-3-methylglutaryl coenzyme a reductase activity of liver microsomes",

abstract = "The specific activity of 3-hydroxy-3-methylglu-taryl coenzyme A (HMG CoA) reductase in microsome-enriched fractions prepared from normal human fetal liver tissue was assessed. The mean specific activity was 0.58 ± 0.18 nmol mev-alonate formed min−1 mg−1 protein. The activity of the enzyme was inhibited by preincubation of microsomes with ATP (4 m.M) and was greatly reduced when microsomes were prepared from tissue homogenized in the presence of NaF (50 HIM). It can be computed that the activity of HMG CoA reductase in human fetal liver microsomes is adequate to provide cholesterol to meet the requirements of the fetal adrenal for steroid precursor provided that cholesterol synthesized in the fetal liver appears in the plasma in the form of low density lipoprotein.",

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AU - Simpson, E. R.

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N2 - The specific activity of 3-hydroxy-3-methylglu-taryl coenzyme A (HMG CoA) reductase in microsome-enriched fractions prepared from normal human fetal liver tissue was assessed. The mean specific activity was 0.58 ± 0.18 nmol mev-alonate formed min−1 mg−1 protein. The activity of the enzyme was inhibited by preincubation of microsomes with ATP (4 m.M) and was greatly reduced when microsomes were prepared from tissue homogenized in the presence of NaF (50 HIM). It can be computed that the activity of HMG CoA reductase in human fetal liver microsomes is adequate to provide cholesterol to meet the requirements of the fetal adrenal for steroid precursor provided that cholesterol synthesized in the fetal liver appears in the plasma in the form of low density lipoprotein.

AB - The specific activity of 3-hydroxy-3-methylglu-taryl coenzyme A (HMG CoA) reductase in microsome-enriched fractions prepared from normal human fetal liver tissue was assessed. The mean specific activity was 0.58 ± 0.18 nmol mev-alonate formed min−1 mg−1 protein. The activity of the enzyme was inhibited by preincubation of microsomes with ATP (4 m.M) and was greatly reduced when microsomes were prepared from tissue homogenized in the presence of NaF (50 HIM). It can be computed that the activity of HMG CoA reductase in human fetal liver microsomes is adequate to provide cholesterol to meet the requirements of the fetal adrenal for steroid precursor provided that cholesterol synthesized in the fetal liver appears in the plasma in the form of low density lipoprotein.

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Synthesis of cholesterol in the human fetus: 3-hydroxy-3-methylglutaryl coenzyme a reductase activity of liver microsomes

Abstract

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