Abstract
Background: In developing muscle, stimulation of mitochondrial biogenesis and mtDNA expansion occur with downregulation of deoxynucleotide synthesis. Results: siRNA silencing of mitochondrial thymidine or deoxyguanosine kinase impacts myotube differentiation causing depletion of mtDNA and of all four deoxynucleotides. Conclusion: Shortage of even a single deoxynucleotide may upset the regulation of all DNA precursors. Significance: Deoxynucleotide analysis in myotubes unveils unexpected outcomes of synthetic enzyme deficiencies.
Original language | English (US) |
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Pages (from-to) | 5624-5635 |
Number of pages | 12 |
Journal | Journal of Biological Chemistry |
Volume | 288 |
Issue number | 8 |
DOIs | |
State | Published - Feb 22 2013 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology