Synthesis of mitochondrial DNA precursors during myogenesis, an analysis in purified C2C12 myotubes

Miriam Frangini, Elisa Franzolin, Francesco Chemello, Paolo Laveder, Chiara Romualdi, Vera Bianchi, Chiara Rampazzo

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Background: In developing muscle, stimulation of mitochondrial biogenesis and mtDNA expansion occur with downregulation of deoxynucleotide synthesis. Results: siRNA silencing of mitochondrial thymidine or deoxyguanosine kinase impacts myotube differentiation causing depletion of mtDNA and of all four deoxynucleotides. Conclusion: Shortage of even a single deoxynucleotide may upset the regulation of all DNA precursors. Significance: Deoxynucleotide analysis in myotubes unveils unexpected outcomes of synthetic enzyme deficiencies.

Original languageEnglish (US)
Pages (from-to)5624-5635
Number of pages12
JournalJournal of Biological Chemistry
Volume288
Issue number8
DOIs
StatePublished - Feb 22 2013
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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