Systematically improved in vitro culture conditions reveal new insights into the reproductive biology of the human parasite Schistosoma mansoni

Jipeng Wang, Rui Chen, James J Collins

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Schistosomes infect over 200 million people. The prodigious egg output of these parasites is the sole driver of pathology due to infection, yet our understanding of sexual reproduction by schistosomes is limited because normal egg production is not sustained for more than a few days in vitro. Here, we describe culture conditions that support schistosome sexual development and sustained egg production in vitro. Female schistosomes rely on continuous pairing with male worms to fuel the maturation of their reproductive organs. Exploiting these new culture conditions, we explore the process of male-stimulated female maturation and demonstrate that physical contact with a male worm, and not insemination, is sufficient to induce female development and the production of viable parthenogenetic haploid embryos. We further report the characterization of a nuclear receptor (NR), which we call Vitellogenic Factor 1 (VF1), that is essential for female sexual development following pairing with a male worm. Taken together, these results provide a platform to study the fascinating sexual biology of these parasites on a molecular level, illuminating new strategies to control schistosome egg production.

Original languageEnglish (US)
Pages (from-to)e3000254
JournalPLoS biology
Volume17
Issue number5
DOIs
StatePublished - May 1 2019

Fingerprint

Schistosoma
Schistosoma mansoni
in vitro culture
Ovum
Parasites
parasites
Biological Sciences
Sexual Development
egg production
sexual development
Insemination
Haploidy
Pathology
Cytoplasmic and Nuclear Receptors
Reproduction
sexual reproduction
Embryonic Structures
haploidy
insemination
gonads

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Systematically improved in vitro culture conditions reveal new insights into the reproductive biology of the human parasite Schistosoma mansoni. / Wang, Jipeng; Chen, Rui; Collins, James J.

In: PLoS biology, Vol. 17, No. 5, 01.05.2019, p. e3000254.

Research output: Contribution to journalArticle

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