Systemic and uterine responses to chronic infusion of estradiol-17β

R. R. Magness, C. R. Parker, C. R. Rosenfeld

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Abstract

Human and ovine pregnancies are associated with increases in plasma levels of estrogens and angiotensin II (ANG II), cardiac output (CO), blood volume (BV), and uterine blood flow (UBF), as well as attenuated ANG II pressor responses. We hypothesized that, in nonpregnant animals, prolonged estradiol- 17β (E2β) treatment would reproduce these endocrine and hemodynamic alterations. Nonpregnant ovariectomized ewes (n = 5) received 5 μg E2β/kg iv followed by 220 μg/day for 14 days. Plasma E2β increased from 36 ± 6 to 269 ± 79 (SE) pg/ml (P < 0.05) during E2β treatment, returning to control values 4 days posttreatment. By 3 days of E2β, mean arterial pressure (MAP) and systemic vascular resistance (SVR) fell 9 ± 1 and 29 ± 1%, whereas heart rate (HR) and CO increased 20 ± 5 and 26 ± 1% (P < 0.05). Stroke volume (SV), BV, and plasma volume were unchanged until 7 days of E2β, with values rising 17 ± 5, 13 ± 3, and 14 ± 4, respectively (P < 0.05). Although MAP remained similarly depressed (-11 ± 1%) during week 2 of E2β, SVR decreased further (-37 ± 3%) and was associated with additional increases (P < 0.05) in CO to 44 ± 5%, reflecting rises in SV (21 ± 2%) but not HR. Increases in BV correlated with rises in CO (r = 0.55) and SV (r = 0.64) but not HR (r = -0.04). Although UBF increased 396 ± 131% 120 min after the E2β bolus (P < 0.05), values returned to control levels by 7 days of E2β treatment. ANG II and plasma renin activity (PRA) increased maximally by 3 days of E2β, from 12 ± 2 pg/ml and 1.28 ± 0.18 ng · ml- 1 · h-1 to 56 ± 29 and 2.75 ± 0.54, respectively (P < 0.05), and returned to control levels at ≥7 days. Pressor responses to infused ANG II (0.115-11.5 μg/min) decreased during E2β treatment, and, despite subsequent decreases in plasma E2β, ANG II, and PRA 2 wk post-E2β, responses remained attenuated (P < 0.05). Prolonged E2β treatment reproduces many cardiovascular alterations associated with pregnancy, including systemic vasodilation and attenuated pressor responses to infused ANG II, but does not maintain elevations in UBF. Furthermore, activation of the renin-angiotensin system via vascular 'underfilling' was reversed after BV expansion.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume265
Issue number5 28-5
StatePublished - 1993

Fingerprint

Angiotensin II
Estradiol
Blood
Blood Volume
Cardiac Output
Plasmas
Stroke Volume
Renin
Heart Rate
Level control
Vascular Resistance
Arterial Pressure
Pregnancy
Plasma Volume
Renin-Angiotensin System
Vasodilation
Angiotensins
Hemodynamics
Blood Vessels
Sheep

Keywords

  • angiotensin II
  • blood pressure
  • blood volume
  • cardiac output
  • pregnancy
  • pressor responses
  • uterine blood flow
  • vascular resistance

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Physiology

Cite this

Systemic and uterine responses to chronic infusion of estradiol-17β. / Magness, R. R.; Parker, C. R.; Rosenfeld, C. R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 265, No. 5 28-5, 1993.

Research output: Contribution to journalArticle

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N2 - Human and ovine pregnancies are associated with increases in plasma levels of estrogens and angiotensin II (ANG II), cardiac output (CO), blood volume (BV), and uterine blood flow (UBF), as well as attenuated ANG II pressor responses. We hypothesized that, in nonpregnant animals, prolonged estradiol- 17β (E2β) treatment would reproduce these endocrine and hemodynamic alterations. Nonpregnant ovariectomized ewes (n = 5) received 5 μg E2β/kg iv followed by 220 μg/day for 14 days. Plasma E2β increased from 36 ± 6 to 269 ± 79 (SE) pg/ml (P < 0.05) during E2β treatment, returning to control values 4 days posttreatment. By 3 days of E2β, mean arterial pressure (MAP) and systemic vascular resistance (SVR) fell 9 ± 1 and 29 ± 1%, whereas heart rate (HR) and CO increased 20 ± 5 and 26 ± 1% (P < 0.05). Stroke volume (SV), BV, and plasma volume were unchanged until 7 days of E2β, with values rising 17 ± 5, 13 ± 3, and 14 ± 4, respectively (P < 0.05). Although MAP remained similarly depressed (-11 ± 1%) during week 2 of E2β, SVR decreased further (-37 ± 3%) and was associated with additional increases (P < 0.05) in CO to 44 ± 5%, reflecting rises in SV (21 ± 2%) but not HR. Increases in BV correlated with rises in CO (r = 0.55) and SV (r = 0.64) but not HR (r = -0.04). Although UBF increased 396 ± 131% 120 min after the E2β bolus (P < 0.05), values returned to control levels by 7 days of E2β treatment. ANG II and plasma renin activity (PRA) increased maximally by 3 days of E2β, from 12 ± 2 pg/ml and 1.28 ± 0.18 ng · ml- 1 · h-1 to 56 ± 29 and 2.75 ± 0.54, respectively (P < 0.05), and returned to control levels at ≥7 days. Pressor responses to infused ANG II (0.115-11.5 μg/min) decreased during E2β treatment, and, despite subsequent decreases in plasma E2β, ANG II, and PRA 2 wk post-E2β, responses remained attenuated (P < 0.05). Prolonged E2β treatment reproduces many cardiovascular alterations associated with pregnancy, including systemic vasodilation and attenuated pressor responses to infused ANG II, but does not maintain elevations in UBF. Furthermore, activation of the renin-angiotensin system via vascular 'underfilling' was reversed after BV expansion.

AB - Human and ovine pregnancies are associated with increases in plasma levels of estrogens and angiotensin II (ANG II), cardiac output (CO), blood volume (BV), and uterine blood flow (UBF), as well as attenuated ANG II pressor responses. We hypothesized that, in nonpregnant animals, prolonged estradiol- 17β (E2β) treatment would reproduce these endocrine and hemodynamic alterations. Nonpregnant ovariectomized ewes (n = 5) received 5 μg E2β/kg iv followed by 220 μg/day for 14 days. Plasma E2β increased from 36 ± 6 to 269 ± 79 (SE) pg/ml (P < 0.05) during E2β treatment, returning to control values 4 days posttreatment. By 3 days of E2β, mean arterial pressure (MAP) and systemic vascular resistance (SVR) fell 9 ± 1 and 29 ± 1%, whereas heart rate (HR) and CO increased 20 ± 5 and 26 ± 1% (P < 0.05). Stroke volume (SV), BV, and plasma volume were unchanged until 7 days of E2β, with values rising 17 ± 5, 13 ± 3, and 14 ± 4, respectively (P < 0.05). Although MAP remained similarly depressed (-11 ± 1%) during week 2 of E2β, SVR decreased further (-37 ± 3%) and was associated with additional increases (P < 0.05) in CO to 44 ± 5%, reflecting rises in SV (21 ± 2%) but not HR. Increases in BV correlated with rises in CO (r = 0.55) and SV (r = 0.64) but not HR (r = -0.04). Although UBF increased 396 ± 131% 120 min after the E2β bolus (P < 0.05), values returned to control levels by 7 days of E2β treatment. ANG II and plasma renin activity (PRA) increased maximally by 3 days of E2β, from 12 ± 2 pg/ml and 1.28 ± 0.18 ng · ml- 1 · h-1 to 56 ± 29 and 2.75 ± 0.54, respectively (P < 0.05), and returned to control levels at ≥7 days. Pressor responses to infused ANG II (0.115-11.5 μg/min) decreased during E2β treatment, and, despite subsequent decreases in plasma E2β, ANG II, and PRA 2 wk post-E2β, responses remained attenuated (P < 0.05). Prolonged E2β treatment reproduces many cardiovascular alterations associated with pregnancy, including systemic vasodilation and attenuated pressor responses to infused ANG II, but does not maintain elevations in UBF. Furthermore, activation of the renin-angiotensin system via vascular 'underfilling' was reversed after BV expansion.

KW - angiotensin II

KW - blood pressure

KW - blood volume

KW - cardiac output

KW - pregnancy

KW - pressor responses

KW - uterine blood flow

KW - vascular resistance

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