T cell antagonism is functionally uncoupled from the 21- and 23-kDa tyrosine-phosphorylated TCR ζ subunits

Lisa A. Pitcher, Pamela S. Ohashi, Nicolai S C Van Oers

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Abstract

The functional effects of altered peptide ligands on T cells is proposed to involve differential intracellular signaling mediated by the 21- and 23-kDa tyrosine-phosphorylated derivatives of the TCR ζ subunit (p21 and p23). To understand the functional contribution of p21 and p23 to T cell development and T cell antagonism, we generated selected TCR ζ transgenic mice maintained on the P14 αβ TCR transgenic line such that p23 or both p21 and p23 were selectively eliminated. Importantly, one line (YF1,2) retains the constitutively tyrosine-phosphorylated p21 in the complete absence of inducible p23. We determined that T cell development was uncoupled from p21 and/or p23. Using a series of agonist, weak agonist, and antagonist peptides, we analyzed the role of each of the phosphorylated forms of TCR ζ on T cell activation and antagonism. In this study, we report that the proliferative responses of αβ P14 T cells to agonist peptides and the inhibition of proliferation resulting from antagonist peptide treatments was functionally uncoupled from p21 and/or p23. These results suggest that the mechanism of T cell antagonism is independent of the two phosphorylated TCR ζ derivatives.

Original languageEnglish (US)
Pages (from-to)845-852
Number of pages8
JournalJournal of Immunology
Volume171
Issue number2
StatePublished - Jul 15 2003

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Tyrosine
T-Lymphocytes
Peptides
Transgenic Mice
Ligands

ASJC Scopus subject areas

  • Immunology

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T cell antagonism is functionally uncoupled from the 21- and 23-kDa tyrosine-phosphorylated TCR ζ subunits. / Pitcher, Lisa A.; Ohashi, Pamela S.; Van Oers, Nicolai S C.

In: Journal of Immunology, Vol. 171, No. 2, 15.07.2003, p. 845-852.

Research output: Contribution to journalArticle

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