T cell-derived B cell growth and differentiation factors. Dichotomy between the responsiveness of B cells from adult and neonatal mice

E. Pure, P. C. Isakson, J. W. Kappler, P. Marrack, P. H. Krammer, E. S. Vitetta

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Abstract

In these studies we have determined the molecular weights of B cell growth factor (BCGF) (< 20,000), and B cell differentiation factors (BCDF) that induce immunoglobulin M (IgM) secretion (BCDF(μ)) (30-60,000) and IgG secretion (BCDF(γ)) (< 20,000). Thus, the molecular weight of BCDF(μ) is distinct from that of BCGF and BCDF(γ); BCGF and BCDF(γ) cannot be distinguished. In addition, BCGF, BCDF(μ), and BCDF(γ) are distinguishable by their presence or absence in different supernatants from a panel of mitogen-induced T cell clones. These results suggest that the three lymphokines are different. This conclusion is supported by their differential biological effect on B cells from adult and neonatal mice. Thus, treatment with anti-Ig induces B cells from adult mice to proliferate and this proliferation is sustained by BCGF. In contrast, even in the presence of BCGF, anti-Ig does not induce B cells from neonatal mice to proliferate. However, BCDF(μ) and BCDF(γ) induce IgM and IgG secretion in B cells, respectively, from both adult and neonatal mice. Thus, mature B cells can both clonally expand and differentiate in response to anti-Ig, BCGF, and BCDF, whereas immature B cells can only differentiate. The poor response of neonatal B cells to anti-Ig and BCGF may partially explain the relative immunoincompetence of immature B cells.

Original languageEnglish (US)
Pages (from-to)600-612
Number of pages13
JournalJournal of Experimental Medicine
Volume157
Issue number2
Publication statusPublished - 1983

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ASJC Scopus subject areas

  • Immunology

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