T cell subsets and sIL-2R/IL-2 levels in patients with glaucoma

Junjie Yang, Rajkumar V. Patil, Hao Yu, Mae Gordon, Martin B. Wax

Research output: Contribution to journalArticle

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Abstract

PURPOSE: We hypothesize that cellular immunity may have a previously unrecognized role in glaucomatous optic neuropathy. The purpose of this study is to analyze subsets of T cells and the levels of cytokine IL-2 and the soluble IL-2 receptor in peripheral blood from patients with normal pressure glaucoma (NPG) or primary open angle glaucoma (POAG) in comparison to age-matched control subjects. METHODS: In this study, 38 patients (20 NPG; 18 POAG) and 19 controls were included. sIL-2R and IL-2 were assayed by ELISA. T cell subsets were analyzed by flow cytometry and lymphocyte proliferation was used to measure the reactive ability of T cells to phytohemagglutinin (PHA). RESULTS: The frequency of CD8+HLA-DR+ lymphocytes were increased in patients with NPG (P = 0.008), and CD3+CD8+ lymphocytes increased in both NPG (P = 0.03) and POAG patients (P = 0.0004). CD5+ lymphocytes were higher only in POAG patients (P = 0.0012). In comparison to controls, the ratio of CD4+/CD8+ lymphocytes was similar in both groups. The mean concentrations of sIL-2R in NPG (P = 0.011) and POAG (P = 0.0023) patients were higher than that found in control subjects although IL-2 concentrations were similar in these groups. In addition, the reactive ability of T lymphocytes to the non-specific reagent (PHA) was reduced significantly in NPG (P = 0.02) and POAG patients (P=0.04). CONCLUSION: The alterations of the cellular immune system in patients with glaucoma support our hypothesis that the immune system may play an important role in the initiation and/or sustainment of glaucomatous optic neuropathy in some patients.

Original languageEnglish (US)
Pages (from-to)421-426
Number of pages6
JournalAmerican Journal of Ophthalmology
Volume131
Issue number4
DOIs
StatePublished - 2001

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T-Lymphocyte Subsets
Glaucoma
Interleukin-2
Pressure
Lymphocytes
Optic Nerve Diseases
Phytohemagglutinins
Immune System
T-Lymphocytes
CD4-CD8 Ratio
Interleukin-2 Receptors
HLA-DR Antigens
Cellular Immunity
Primary Open Angle Glaucoma
Flow Cytometry
Enzyme-Linked Immunosorbent Assay
Cytokines

ASJC Scopus subject areas

  • Ophthalmology

Cite this

T cell subsets and sIL-2R/IL-2 levels in patients with glaucoma. / Yang, Junjie; Patil, Rajkumar V.; Yu, Hao; Gordon, Mae; Wax, Martin B.

In: American Journal of Ophthalmology, Vol. 131, No. 4, 2001, p. 421-426.

Research output: Contribution to journalArticle

Yang, Junjie ; Patil, Rajkumar V. ; Yu, Hao ; Gordon, Mae ; Wax, Martin B. / T cell subsets and sIL-2R/IL-2 levels in patients with glaucoma. In: American Journal of Ophthalmology. 2001 ; Vol. 131, No. 4. pp. 421-426.
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