T helper cell dysfunction in systemic lupus erythematosus (SLE): Relation to disease activity

Bonnie L. Bermas, Michelle Petri, Daniel Goldman, Barbara Mittleman, Matthew W. Miller, Naomi I. Stocks, Charles S. Via, Gene M. Shearer

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Patients with systemic lupus erythematosus (SLE) are known to have defects in both humoral and cellular immunity. The significance of defective T cell-mediated immunity and its relationship to disease activity have not been clearly established. We studied in vitro T helper cell (Th) function in 150 SLE outpatients and correlated Th function with validated measures of disease activity. Interleukin 2 (IL-2) production by peripheral blood mononuclear cells (PBMC) was measured after stimulation with the recall antigens influenza A virus (FLU) and tetanus toxoid (TET), irradiated allogeneic peripheral blood mononuclear cells (ALLO), and phytohemagglutinin (PHA). We observed three patterns of Th response: (1) 76 of 150 (50%) of patients responded to the recall antigens FLU and/or TET, ALLO, and PHA; (2) 62 of 150 (42%) of patients did not respond to recall antigens but responded to ALLO and PHA; and (3) 12 of 150 (8%) of patients did not respond to either recall antigens or ALLO antigens. This diminished T cell function was correlated with higher disease activity as measured by four scales of clinical activity, such that individuals who exhibited more in vitro immune dysfunction presented with significant increases in their clinical activity indicies. The alterations in T cell function could not be accounted for by medication doses alone. Thus, SLE patients have multiple distinct defects at the level of the Th cell which are associated with clinical measures of disease activity.

Original languageEnglish (US)
Pages (from-to)169-177
Number of pages9
JournalJournal of Clinical Immunology
Volume14
Issue number3
DOIs
StatePublished - May 1 1994

Fingerprint

Helper-Inducer T-Lymphocytes
Systemic Lupus Erythematosus
Antigens
Phytohemagglutinins
Tetanus Toxoid
T-Lymphocytes
Cellular Immunity
Blood Cells
Th1 Cells
Influenza A virus
Humoral Immunity
Interleukin-2
Outpatients
In Vitro Techniques

Keywords

  • Interleukin-2
  • Lupus Activity Index
  • systemic lupus erythematosus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Bermas, B. L., Petri, M., Goldman, D., Mittleman, B., Miller, M. W., Stocks, N. I., ... Shearer, G. M. (1994). T helper cell dysfunction in systemic lupus erythematosus (SLE): Relation to disease activity. Journal of Clinical Immunology, 14(3), 169-177. https://doi.org/10.1007/BF01533366

T helper cell dysfunction in systemic lupus erythematosus (SLE) : Relation to disease activity. / Bermas, Bonnie L.; Petri, Michelle; Goldman, Daniel; Mittleman, Barbara; Miller, Matthew W.; Stocks, Naomi I.; Via, Charles S.; Shearer, Gene M.

In: Journal of Clinical Immunology, Vol. 14, No. 3, 01.05.1994, p. 169-177.

Research output: Contribution to journalArticle

Bermas, BL, Petri, M, Goldman, D, Mittleman, B, Miller, MW, Stocks, NI, Via, CS & Shearer, GM 1994, 'T helper cell dysfunction in systemic lupus erythematosus (SLE): Relation to disease activity', Journal of Clinical Immunology, vol. 14, no. 3, pp. 169-177. https://doi.org/10.1007/BF01533366
Bermas, Bonnie L. ; Petri, Michelle ; Goldman, Daniel ; Mittleman, Barbara ; Miller, Matthew W. ; Stocks, Naomi I. ; Via, Charles S. ; Shearer, Gene M. / T helper cell dysfunction in systemic lupus erythematosus (SLE) : Relation to disease activity. In: Journal of Clinical Immunology. 1994 ; Vol. 14, No. 3. pp. 169-177.
@article{829a4c9b0a4e4bde843b65d5f2d230de,
title = "T helper cell dysfunction in systemic lupus erythematosus (SLE): Relation to disease activity",
abstract = "Patients with systemic lupus erythematosus (SLE) are known to have defects in both humoral and cellular immunity. The significance of defective T cell-mediated immunity and its relationship to disease activity have not been clearly established. We studied in vitro T helper cell (Th) function in 150 SLE outpatients and correlated Th function with validated measures of disease activity. Interleukin 2 (IL-2) production by peripheral blood mononuclear cells (PBMC) was measured after stimulation with the recall antigens influenza A virus (FLU) and tetanus toxoid (TET), irradiated allogeneic peripheral blood mononuclear cells (ALLO), and phytohemagglutinin (PHA). We observed three patterns of Th response: (1) 76 of 150 (50{\%}) of patients responded to the recall antigens FLU and/or TET, ALLO, and PHA; (2) 62 of 150 (42{\%}) of patients did not respond to recall antigens but responded to ALLO and PHA; and (3) 12 of 150 (8{\%}) of patients did not respond to either recall antigens or ALLO antigens. This diminished T cell function was correlated with higher disease activity as measured by four scales of clinical activity, such that individuals who exhibited more in vitro immune dysfunction presented with significant increases in their clinical activity indicies. The alterations in T cell function could not be accounted for by medication doses alone. Thus, SLE patients have multiple distinct defects at the level of the Th cell which are associated with clinical measures of disease activity.",
keywords = "Interleukin-2, Lupus Activity Index, systemic lupus erythematosus",
author = "Bermas, {Bonnie L.} and Michelle Petri and Daniel Goldman and Barbara Mittleman and Miller, {Matthew W.} and Stocks, {Naomi I.} and Via, {Charles S.} and Shearer, {Gene M.}",
year = "1994",
month = "5",
day = "1",
doi = "10.1007/BF01533366",
language = "English (US)",
volume = "14",
pages = "169--177",
journal = "Journal of Clinical Immunology",
issn = "0271-9142",
publisher = "Springer New York",
number = "3",

}

TY - JOUR

T1 - T helper cell dysfunction in systemic lupus erythematosus (SLE)

T2 - Relation to disease activity

AU - Bermas, Bonnie L.

AU - Petri, Michelle

AU - Goldman, Daniel

AU - Mittleman, Barbara

AU - Miller, Matthew W.

AU - Stocks, Naomi I.

AU - Via, Charles S.

AU - Shearer, Gene M.

PY - 1994/5/1

Y1 - 1994/5/1

N2 - Patients with systemic lupus erythematosus (SLE) are known to have defects in both humoral and cellular immunity. The significance of defective T cell-mediated immunity and its relationship to disease activity have not been clearly established. We studied in vitro T helper cell (Th) function in 150 SLE outpatients and correlated Th function with validated measures of disease activity. Interleukin 2 (IL-2) production by peripheral blood mononuclear cells (PBMC) was measured after stimulation with the recall antigens influenza A virus (FLU) and tetanus toxoid (TET), irradiated allogeneic peripheral blood mononuclear cells (ALLO), and phytohemagglutinin (PHA). We observed three patterns of Th response: (1) 76 of 150 (50%) of patients responded to the recall antigens FLU and/or TET, ALLO, and PHA; (2) 62 of 150 (42%) of patients did not respond to recall antigens but responded to ALLO and PHA; and (3) 12 of 150 (8%) of patients did not respond to either recall antigens or ALLO antigens. This diminished T cell function was correlated with higher disease activity as measured by four scales of clinical activity, such that individuals who exhibited more in vitro immune dysfunction presented with significant increases in their clinical activity indicies. The alterations in T cell function could not be accounted for by medication doses alone. Thus, SLE patients have multiple distinct defects at the level of the Th cell which are associated with clinical measures of disease activity.

AB - Patients with systemic lupus erythematosus (SLE) are known to have defects in both humoral and cellular immunity. The significance of defective T cell-mediated immunity and its relationship to disease activity have not been clearly established. We studied in vitro T helper cell (Th) function in 150 SLE outpatients and correlated Th function with validated measures of disease activity. Interleukin 2 (IL-2) production by peripheral blood mononuclear cells (PBMC) was measured after stimulation with the recall antigens influenza A virus (FLU) and tetanus toxoid (TET), irradiated allogeneic peripheral blood mononuclear cells (ALLO), and phytohemagglutinin (PHA). We observed three patterns of Th response: (1) 76 of 150 (50%) of patients responded to the recall antigens FLU and/or TET, ALLO, and PHA; (2) 62 of 150 (42%) of patients did not respond to recall antigens but responded to ALLO and PHA; and (3) 12 of 150 (8%) of patients did not respond to either recall antigens or ALLO antigens. This diminished T cell function was correlated with higher disease activity as measured by four scales of clinical activity, such that individuals who exhibited more in vitro immune dysfunction presented with significant increases in their clinical activity indicies. The alterations in T cell function could not be accounted for by medication doses alone. Thus, SLE patients have multiple distinct defects at the level of the Th cell which are associated with clinical measures of disease activity.

KW - Interleukin-2

KW - Lupus Activity Index

KW - systemic lupus erythematosus

UR - http://www.scopus.com/inward/record.url?scp=0028303417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028303417&partnerID=8YFLogxK

U2 - 10.1007/BF01533366

DO - 10.1007/BF01533366

M3 - Article

C2 - 7929693

AN - SCOPUS:0028303417

VL - 14

SP - 169

EP - 177

JO - Journal of Clinical Immunology

JF - Journal of Clinical Immunology

SN - 0271-9142

IS - 3

ER -