T lymphocyte modification with the UTA microporous polyurethane vascular prosthesis: In vivo studies in rats

Y. Marois, R. Roy, M. Marois, R. G. Guidoin, W. W. Von Maltzahn, R. Kowligi, R. C. Eberhart

Research output: Contribution to journalArticle

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Abstract

Sequential quantification of blood T cell subsets by immunocytofluorometry was used to investigate the immune response of microporous polyurethane vascular prostheses after intraperitoneal implantation in rats. The experimental prosthesis, as developed by the University of Texas-Arlington group (UTA), and the Mitrathane® prosthesis, as developed by Matrix Med., were implanted for 1, 2 and 6 weeks and compared with ePTFE and wounded rats without prostheses (control group). The implants were examined for histopathology by light microscopy. The percentages of CD4-(helper) and CD8-(suppressor) bearing cells of the PTFE group were significantly lower (p < 0.05) than the control group 1 week post-implantation. The UTA and the Mitrathane® grafts exhibited a significant decrease in both T cell subsets at 1 week, and CD4-bearing cells at 2 weeks. At 6 weeks, T cell subsets were similar among all groups. The ratio of CD4/CD8- cells was similar among all groups except for the PTFE group, which was lower than the control group after 1 week. Histological examination of Mitrathane® and UTA grafts showed an acute phase of inflammation which lasted at least 2 weeks. Some foreign body giant cells (FBGC) were present 2 weeks post-implantation, and encapsulation was greater than that observed with PTFE grafts. On the other hand, PTFE grafts exhibited a different pattern of inflammation compared to polyurethane grafts. PTFE implants exhibited a moderate chronic inflammatory response for the first week, as shown by the formation of FBGC. At 2 and 6 weeks, the grafts were encapsulated by a thin layer of collagenous tissue and FBGC were still present around the implants, mostly located in contact with the reinforcing mesh. Polyurethane vascular grafts apparently modified peripheral T lymphocyte populations and induced an acute phase of inflammation which lasted longer than the PTFE graft.

Original languageEnglish (US)
Pages (from-to)141-149
Number of pages9
JournalClinical and Investigative Medicine
Volume15
Issue number2
StatePublished - 1992

Fingerprint

Blood Vessel Prosthesis
Polyurethanes
Polytetrafluoroethylene
T-Lymphocytes
Transplants
Foreign Body Giant Cells
T-Lymphocyte Subsets
Prostheses and Implants
Inflammation
Control Groups
CD4-CD8 Ratio
Blood Vessels
Microscopy
Blood Cells
Light

Keywords

  • immunology
  • inflammatory reaction
  • polyurethane grafts
  • T cell subsets
  • T cells

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Marois, Y., Roy, R., Marois, M., Guidoin, R. G., Von Maltzahn, W. W., Kowligi, R., & Eberhart, R. C. (1992). T lymphocyte modification with the UTA microporous polyurethane vascular prosthesis: In vivo studies in rats. Clinical and Investigative Medicine, 15(2), 141-149.

T lymphocyte modification with the UTA microporous polyurethane vascular prosthesis : In vivo studies in rats. / Marois, Y.; Roy, R.; Marois, M.; Guidoin, R. G.; Von Maltzahn, W. W.; Kowligi, R.; Eberhart, R. C.

In: Clinical and Investigative Medicine, Vol. 15, No. 2, 1992, p. 141-149.

Research output: Contribution to journalArticle

Marois, Y, Roy, R, Marois, M, Guidoin, RG, Von Maltzahn, WW, Kowligi, R & Eberhart, RC 1992, 'T lymphocyte modification with the UTA microporous polyurethane vascular prosthesis: In vivo studies in rats', Clinical and Investigative Medicine, vol. 15, no. 2, pp. 141-149.
Marois Y, Roy R, Marois M, Guidoin RG, Von Maltzahn WW, Kowligi R et al. T lymphocyte modification with the UTA microporous polyurethane vascular prosthesis: In vivo studies in rats. Clinical and Investigative Medicine. 1992;15(2):141-149.
Marois, Y. ; Roy, R. ; Marois, M. ; Guidoin, R. G. ; Von Maltzahn, W. W. ; Kowligi, R. ; Eberhart, R. C. / T lymphocyte modification with the UTA microporous polyurethane vascular prosthesis : In vivo studies in rats. In: Clinical and Investigative Medicine. 1992 ; Vol. 15, No. 2. pp. 141-149.
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abstract = "Sequential quantification of blood T cell subsets by immunocytofluorometry was used to investigate the immune response of microporous polyurethane vascular prostheses after intraperitoneal implantation in rats. The experimental prosthesis, as developed by the University of Texas-Arlington group (UTA), and the Mitrathane{\circledR} prosthesis, as developed by Matrix Med., were implanted for 1, 2 and 6 weeks and compared with ePTFE and wounded rats without prostheses (control group). The implants were examined for histopathology by light microscopy. The percentages of CD4-(helper) and CD8-(suppressor) bearing cells of the PTFE group were significantly lower (p < 0.05) than the control group 1 week post-implantation. The UTA and the Mitrathane{\circledR} grafts exhibited a significant decrease in both T cell subsets at 1 week, and CD4-bearing cells at 2 weeks. At 6 weeks, T cell subsets were similar among all groups. The ratio of CD4/CD8- cells was similar among all groups except for the PTFE group, which was lower than the control group after 1 week. Histological examination of Mitrathane{\circledR} and UTA grafts showed an acute phase of inflammation which lasted at least 2 weeks. Some foreign body giant cells (FBGC) were present 2 weeks post-implantation, and encapsulation was greater than that observed with PTFE grafts. On the other hand, PTFE grafts exhibited a different pattern of inflammation compared to polyurethane grafts. PTFE implants exhibited a moderate chronic inflammatory response for the first week, as shown by the formation of FBGC. At 2 and 6 weeks, the grafts were encapsulated by a thin layer of collagenous tissue and FBGC were still present around the implants, mostly located in contact with the reinforcing mesh. Polyurethane vascular grafts apparently modified peripheral T lymphocyte populations and induced an acute phase of inflammation which lasted longer than the PTFE graft.",
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