TALEN-mediated somatic mutagenesis in murine models of cancer

Shuyuan Zhang, Lin Li, Sara L. Kendrick, Robert D. Gerard, Hao Zhu

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Cancer genome sequencing has identified numerous somatic mutations whose biologic relevance is uncertain. In this study, we used genome-editing tools to create and analyze targeted somatic mutations in murine models of liver cancer. Transcription activator-like effector nucleases (TALEN) were designed against β-catenin (Ctnnb1) and adenomatous polyposis coli (Apc), two commonly mutated genes in hepatocellular carcinoma (HCC), to generate isogenic HCC cell lines. Both mutant cell lines exhibited evidence of Wnt pathway dysregulation. We asked whether these TALENs could create targeted somatic mutations after hydrodynamic transfection into mouse liver. TALENs targeting β-catenin promoted endogenous HCC carrying the intended gain-of-function mutations. However, TALENs targeting Apc were not as efficient in inducing in vivo homozygous loss-of-function mutations. We hypothesized that hepatocyte polyploidy might be protective against TALEN-induced loss of heterozygosity, and indeed Apc gene editing was less efficient in tetraploid than in diploid hepatocytes. To increase efficiency, we administered adenoviral Apc TALENs and found that we could achieve a higher mutagenesis rate in vivo. Our results demonstrate that genome-editing tools can enable the in vivo study of cancer genes and faithfully recapitulate the mosaic nature of mutagenesis in mouse cancer models.

Original languageEnglish (US)
Pages (from-to)5311-5321
Number of pages11
JournalCancer Research
Volume74
Issue number18
DOIs
Publication statusPublished - Jul 28 2014

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ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

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