Targeted germline modifications in rats using CRISPR/Cas9 and spermatogonial stem cells

Karen M. Chapman, Gerardo A. Medrano, Priscilla Jaichander, Jaideep Chaudhary, Alexandra E. Waits, Marcelo A. Nobrega, James M. Hotaling, Carole Ober, Kent F. Hamra

Research output: Contribution to journalArticle

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Abstract

Organisms with targeted genomic modifications are efficiently produced by gene editing in embryos using CRISPR/Cas9 RNA-guided DNA endonuclease. Here, to facilitate germline editing in rats, we used CRISPR/Cas9 to catalyze targeted genomic mutations in rat spermatogonial stem cell cultures. CRISPR/Cas9-modified spermatogonia regenerated spermatogenesis and displayed long-term sperm-forming potential following transplantation into rat testes. Targeted germline mutations in Epsti1 and Erbb3 were vertically transmitted from recipients toexclusively generate "pure," non-mosaic mutant progeny. Epsti1 mutant rats were produced with or without genetic selection of donor spermatogonia. Monoclonal enrichment of Erbb3 null germlines unmasked recessive spermatogenesis defects in culture that were buffered in recipients, yielding mutant progeny isogenic at targeted alleles. Thus, spermatogonial gene editing with CRISPR/Cas9 provided a platform for generating targeted germline mutations in rats and for studying spermatogenesis.

Original languageEnglish (US)
Pages (from-to)1828-1835
Number of pages8
JournalCell Reports
Volume10
Issue number11
DOIs
StatePublished - Mar 24 2015

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Clustered Regularly Interspaced Short Palindromic Repeats
Stem cells
Rats
Stem Cells
Spermatogenesis
Spermatogonia
Germ-Line Mutation
Genes
Genetic Selection
Deoxyribonuclease I
Cell culture
Spermatozoa
Testis
Embryonic Structures
Cell Culture Techniques
Transplantation
Alleles
RNA
Defects
Mutation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Chapman, K. M., Medrano, G. A., Jaichander, P., Chaudhary, J., Waits, A. E., Nobrega, M. A., ... Hamra, K. F. (2015). Targeted germline modifications in rats using CRISPR/Cas9 and spermatogonial stem cells. Cell Reports, 10(11), 1828-1835. https://doi.org/10.1016/j.celrep.2015.02.040

Targeted germline modifications in rats using CRISPR/Cas9 and spermatogonial stem cells. / Chapman, Karen M.; Medrano, Gerardo A.; Jaichander, Priscilla; Chaudhary, Jaideep; Waits, Alexandra E.; Nobrega, Marcelo A.; Hotaling, James M.; Ober, Carole; Hamra, Kent F.

In: Cell Reports, Vol. 10, No. 11, 24.03.2015, p. 1828-1835.

Research output: Contribution to journalArticle

Chapman, KM, Medrano, GA, Jaichander, P, Chaudhary, J, Waits, AE, Nobrega, MA, Hotaling, JM, Ober, C & Hamra, KF 2015, 'Targeted germline modifications in rats using CRISPR/Cas9 and spermatogonial stem cells', Cell Reports, vol. 10, no. 11, pp. 1828-1835. https://doi.org/10.1016/j.celrep.2015.02.040
Chapman KM, Medrano GA, Jaichander P, Chaudhary J, Waits AE, Nobrega MA et al. Targeted germline modifications in rats using CRISPR/Cas9 and spermatogonial stem cells. Cell Reports. 2015 Mar 24;10(11):1828-1835. https://doi.org/10.1016/j.celrep.2015.02.040
Chapman, Karen M. ; Medrano, Gerardo A. ; Jaichander, Priscilla ; Chaudhary, Jaideep ; Waits, Alexandra E. ; Nobrega, Marcelo A. ; Hotaling, James M. ; Ober, Carole ; Hamra, Kent F. / Targeted germline modifications in rats using CRISPR/Cas9 and spermatogonial stem cells. In: Cell Reports. 2015 ; Vol. 10, No. 11. pp. 1828-1835.
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