Targeted investigation of the neandertal genome by array-based sequence capture

Hernán A. Burbano, Emily Hodges, Richard E. Green, Adrian W. Briggs, Johannes Krause, Matthias Meyer, Jeffrey M. Good, Tomislav Maricic, Philip L.F. Johnson, Zhenyu Xuan, Michelle Rooks, Arindam Bhattacharjee, Leonardo Brizuela, Frank W. Albert, Marco De La Rasilla, Javier Fortea, Antonio Rosas, Michael Lachmann, Gregory J. Hannon, Svante Pääbo

Research output: Contribution to journalArticle

188 Citations (Scopus)

Abstract

It is now possible to perform whole-genome shotgun sequencing as well as capture of specific genomic regions for extinct organisms. However, targeted resequencing of large parts of nuclear genomes has yet to be demonstrated for ancient DNA. Here we show that hybridization capture on microarrays can successfully recover more than a megabase of target regions from Neandertal DNA even in the presence of ∼99.8% microbial DNA. Using this approach, we have sequenced -14,000 protein-coding positions inferred to have changed on the human lineage since the last common ancestor shared with chimpanzees. By generating the sequence of one Neandertal and 50 present-day humans at these positions, we have identified 88 amino acid substitutions that have become fixed in humans since our divergence from the Neandertals.

Original languageEnglish (US)
Pages (from-to)723-725
Number of pages3
JournalScience
Volume328
Issue number5979
DOIs
StatePublished - May 7 2010
Externally publishedYes

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Neanderthals
Genome
Pan troglodytes
DNA
Firearms
Amino Acid Substitution
Proteins

ASJC Scopus subject areas

  • General

Cite this

Burbano, H. A., Hodges, E., Green, R. E., Briggs, A. W., Krause, J., Meyer, M., ... Pääbo, S. (2010). Targeted investigation of the neandertal genome by array-based sequence capture. Science, 328(5979), 723-725. https://doi.org/10.1126/science.1188046

Targeted investigation of the neandertal genome by array-based sequence capture. / Burbano, Hernán A.; Hodges, Emily; Green, Richard E.; Briggs, Adrian W.; Krause, Johannes; Meyer, Matthias; Good, Jeffrey M.; Maricic, Tomislav; Johnson, Philip L.F.; Xuan, Zhenyu; Rooks, Michelle; Bhattacharjee, Arindam; Brizuela, Leonardo; Albert, Frank W.; De La Rasilla, Marco; Fortea, Javier; Rosas, Antonio; Lachmann, Michael; Hannon, Gregory J.; Pääbo, Svante.

In: Science, Vol. 328, No. 5979, 07.05.2010, p. 723-725.

Research output: Contribution to journalArticle

Burbano, HA, Hodges, E, Green, RE, Briggs, AW, Krause, J, Meyer, M, Good, JM, Maricic, T, Johnson, PLF, Xuan, Z, Rooks, M, Bhattacharjee, A, Brizuela, L, Albert, FW, De La Rasilla, M, Fortea, J, Rosas, A, Lachmann, M, Hannon, GJ & Pääbo, S 2010, 'Targeted investigation of the neandertal genome by array-based sequence capture', Science, vol. 328, no. 5979, pp. 723-725. https://doi.org/10.1126/science.1188046
Burbano HA, Hodges E, Green RE, Briggs AW, Krause J, Meyer M et al. Targeted investigation of the neandertal genome by array-based sequence capture. Science. 2010 May 7;328(5979):723-725. https://doi.org/10.1126/science.1188046
Burbano, Hernán A. ; Hodges, Emily ; Green, Richard E. ; Briggs, Adrian W. ; Krause, Johannes ; Meyer, Matthias ; Good, Jeffrey M. ; Maricic, Tomislav ; Johnson, Philip L.F. ; Xuan, Zhenyu ; Rooks, Michelle ; Bhattacharjee, Arindam ; Brizuela, Leonardo ; Albert, Frank W. ; De La Rasilla, Marco ; Fortea, Javier ; Rosas, Antonio ; Lachmann, Michael ; Hannon, Gregory J. ; Pääbo, Svante. / Targeted investigation of the neandertal genome by array-based sequence capture. In: Science. 2010 ; Vol. 328, No. 5979. pp. 723-725.
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