TY - GEN
T1 - Targeted molecular ultrasound therapy improves chemotherapeutic drug delivery in cancer cells
AU - Sorace, Anna G.
AU - Saini, Reshu
AU - Mahoney, Marshall J.
AU - Hoyt, Kenneth
PY - 2012/12/1
Y1 - 2012/12/1
N2 - Microbubble (MB) mediated ultrasound (US) therapy has been shown to non-invasively increase drug uptake through increasing cell membrane permeability and vascular extravasation. Increasing MB-cell interaction through targeted receptors overexpressed in the tumor vasculature promises to increase effectiveness of MB-mediated US therapy. 2LMP breast cancer cells were plated on acoustically transparent tissue culture plates. Using a model system which allows receptor density modulation, molecular US therapy was analyzed for anticancer effects in vitro. Drug (paclitaxel) effectiveness in combination with traditional MB-mediated US therapy or molecular US therapy was analyzed using flow cytometry and ATPlite assays. It was shown that molecular US therapy significantly increases anticancer effects by 25% compared to traditional MB-mediated US therapy (P < 0.001). This preclinical approach has potential to increase localized delivery through a targeted methodology, thereby potentially decreasing systemically toxicity. This study demonstrates that MBs targeted to cellular receptors are a promising addition to MB-mediated US therapy.
AB - Microbubble (MB) mediated ultrasound (US) therapy has been shown to non-invasively increase drug uptake through increasing cell membrane permeability and vascular extravasation. Increasing MB-cell interaction through targeted receptors overexpressed in the tumor vasculature promises to increase effectiveness of MB-mediated US therapy. 2LMP breast cancer cells were plated on acoustically transparent tissue culture plates. Using a model system which allows receptor density modulation, molecular US therapy was analyzed for anticancer effects in vitro. Drug (paclitaxel) effectiveness in combination with traditional MB-mediated US therapy or molecular US therapy was analyzed using flow cytometry and ATPlite assays. It was shown that molecular US therapy significantly increases anticancer effects by 25% compared to traditional MB-mediated US therapy (P < 0.001). This preclinical approach has potential to increase localized delivery through a targeted methodology, thereby potentially decreasing systemically toxicity. This study demonstrates that MBs targeted to cellular receptors are a promising addition to MB-mediated US therapy.
UR - http://www.scopus.com/inward/record.url?scp=84882372036&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84882372036&partnerID=8YFLogxK
U2 - 10.1109/ULTSYM.2012.0106
DO - 10.1109/ULTSYM.2012.0106
M3 - Conference contribution
AN - SCOPUS:84882372036
SN - 9781467345613
T3 - IEEE International Ultrasonics Symposium, IUS
SP - 429
EP - 432
BT - 2012 IEEE International Ultrasonics Symposium, IUS 2012
T2 - 2012 IEEE International Ultrasonics Symposium, IUS 2012
Y2 - 7 October 2012 through 10 October 2012
ER -