Targeting vascular endothelium with avidin microbubbles

Grzegorz Korpanty, Paul A. Grayburn, Ralph V. Shohet, Rolf A. Brekken

Research output: Contribution to journalArticle

78 Scopus citations

Abstract

Targeting microbubbles (MBs) to specific vascular beds enables contrast ultrasound to be used for molecular imaging. There are several methods for attaching targeting moieties to the surface of MBs. In the present study, we demonstrate that avidin (Av) can be incorporated into the shell of perfluorocarbon-exposed sonicated dextrose albumin (PESDA) MBs (Av-PESDA-MBs) and serve as an anchor that links Av-PESDA-MBs to biotinylated monoclonal antibodies (mAbs). This novel linking strategy was used to conjugate Av-PESDA-MBs to mAbs specific for endoglin (CD105) or a control IgG. MBs targeted to CD105 specifically bound to endothelial cells, but not to fibroblasts, in vitro but Av-PESDA-MBs conjugated with the control IgG did not specifically target either cell type. We conclude that Av-PESDA-MBs represent a novel and attractive tool to conjugate MBs with biotinylated mAbs for the purposes of vascular targeting and molecular imaging.

Original languageEnglish (US)
Pages (from-to)1279-1283
Number of pages5
JournalUltrasound in Medicine and Biology
Volume31
Issue number9
DOIs
Publication statusPublished - Sep 2005

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Keywords

  • Avidin-biotin system
  • Contrast ultrasound
  • Molecular imaging
  • Targeted microbubbles
  • Vascular endothelium

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

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