Vaccination of mice with activated, autoantigen reactive CD4+ T cells induces protection from a variety of experimental autoimmune diseases. Although the mechanisms of this protection are not completely known, there is evidence that TCV induces CDS+ regulatory T cells that specifically recognize pathogenic CD4+ T cells. We now show that during TCV, populations of CD8+ T cells emerge which preferentially recognize activated autologous CD4+ T cells in a Qa-1 restricted, TCR Vβ specific manner. To analyze the specificity of these CD8+cells at a clonal level, we generated CD8+ T cell hybridoma clones from B10.PL mice vaccinated with a syngeneic, myelin basic protein specific, CD4+ Vβ8+ T cell clone. Hybridomas were screened for the ability to respond to a syngeneic CD4+Vβ8+ and not CD4+ Vβ6+ cell lines. Two stable Vβ specific hybridoma clones were derived. The first hybridoma is stimulated by the inducing clone as well as other Vβ8+ antigen or superantigen-specific, syngeneic T cells but is not stimulated by four different syngeneic Vβ6+ T cells. A second hybridoma has a similar pattern of TCR Vβ reactivity but differs in its fine specificity. The responses of both hybridomas are inhibited by antiQa-1a but not by anti-Qa-1b sera nor by monoclonal antibodies to framework regions of MHC class I-a. These data show that TCV induces Qa-1 restricted, TCR Vβ specific CD8+ T cell clones which could function in vivo to regulate autoimmunity.
|Original language||English (US)|
|State||Published - Mar 20 1998|
ASJC Scopus subject areas
- Molecular Biology