TCR Vβ specific, Qa-1 restricted CD8+ T hybridoma clones generated from T Cell Vaccinated (TCV)

Mice H. Jiang, H. Kashleva, B. Yu, J. Forman, L. Flaherty, B. Pernis, N. Braunstein, L. Chess

Research output: Contribution to journalArticle

Abstract

Vaccination of mice with activated, autoantigen reactive CD4+ T cells induces protection from a variety of experimental autoimmune diseases. Although the mechanisms of this protection are not completely known, there is evidence that TCV induces CDS+ regulatory T cells that specifically recognize pathogenic CD4+ T cells. We now show that during TCV, populations of CD8+ T cells emerge which preferentially recognize activated autologous CD4+ T cells in a Qa-1 restricted, TCR Vβ specific manner. To analyze the specificity of these CD8+cells at a clonal level, we generated CD8+ T cell hybridoma clones from B10.PL mice vaccinated with a syngeneic, myelin basic protein specific, CD4+ Vβ8+ T cell clone. Hybridomas were screened for the ability to respond to a syngeneic CD4+Vβ8+ and not CD4+ Vβ6+ cell lines. Two stable Vβ specific hybridoma clones were derived. The first hybridoma is stimulated by the inducing clone as well as other Vβ8+ antigen or superantigen-specific, syngeneic T cells but is not stimulated by four different syngeneic Vβ6+ T cells. A second hybridoma has a similar pattern of TCR Vβ reactivity but differs in its fine specificity. The responses of both hybridomas are inhibited by antiQa-1a but not by anti-Qa-1b sera nor by monoclonal antibodies to framework regions of MHC class I-a. These data show that TCV induces Qa-1 restricted, TCR Vβ specific CD8+ T cell clones which could function in vivo to regulate autoimmunity.

Original languageEnglish (US)
Pages (from-to)A1092
JournalFASEB Journal
Volume12
Issue number5
StatePublished - Mar 20 1998

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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  • Cite this

    Jiang, M. H., Kashleva, H., Yu, B., Forman, J., Flaherty, L., Pernis, B., Braunstein, N., & Chess, L. (1998). TCR Vβ specific, Qa-1 restricted CD8+ T hybridoma clones generated from T Cell Vaccinated (TCV). FASEB Journal, 12(5), A1092.