TY - JOUR
T1 - Tear film lipids
AU - Butovich, Igor A.
N1 - Funding Information:
This work has been supported in part by an NIH Grant R01EY019480 , an unrestricted grant from the Research to Prevent Blindness Foundation, New York, New York , and the Department of Ophthalmology of the UT Southwestern Medical Center .
PY - 2013/12
Y1 - 2013/12
N2 - Human meibomian gland secretions (MGS, or meibum) are formed from a complex mixture of lipids of different classes such as wax esters, cholesteryl esters, (O-acyl)-ω-hydroxy fatty acids (OAHFA) and their esters, acylglycerols, diacylated diols, free fatty acids, cholesterol, and a smaller amount of other polar and nonpolar lipids, whose chemical nature and the very presence in MGS have been a matter of intense debates. The purpose of this review is to discuss recent results that were obtained using different experimental techniques, estimate limitations of their usability, and discuss their biochemical, biophysical, and physiological implications. To create a lipid map of MGS and tears, the results obtained in the author's laboratory were integrated with available information on chemical composition of MGS andtears. The most informative approaches that are available today to researchers, such as HPLC-MS, GC-MS, and proton NMR, are discussed in details. A map of the meibomian lipidome (as it is seen in reverse phase liquid chromatography/mass spectrometry experiments) is presented. Directions of future efforts in the area are outlined.
AB - Human meibomian gland secretions (MGS, or meibum) are formed from a complex mixture of lipids of different classes such as wax esters, cholesteryl esters, (O-acyl)-ω-hydroxy fatty acids (OAHFA) and their esters, acylglycerols, diacylated diols, free fatty acids, cholesterol, and a smaller amount of other polar and nonpolar lipids, whose chemical nature and the very presence in MGS have been a matter of intense debates. The purpose of this review is to discuss recent results that were obtained using different experimental techniques, estimate limitations of their usability, and discuss their biochemical, biophysical, and physiological implications. To create a lipid map of MGS and tears, the results obtained in the author's laboratory were integrated with available information on chemical composition of MGS andtears. The most informative approaches that are available today to researchers, such as HPLC-MS, GC-MS, and proton NMR, are discussed in details. A map of the meibomian lipidome (as it is seen in reverse phase liquid chromatography/mass spectrometry experiments) is presented. Directions of future efforts in the area are outlined.
KW - Chromatography
KW - Lipidomics
KW - Lipids
KW - Mass spectrometry
KW - Meibomian gland secretions
KW - Meibum
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U2 - 10.1016/j.exer.2013.05.010
DO - 10.1016/j.exer.2013.05.010
M3 - Review article
C2 - 23769846
AN - SCOPUS:84888130514
SN - 0014-4835
VL - 117
SP - 4
EP - 27
JO - Experimental Eye Research
JF - Experimental Eye Research
ER -