Telomerase reactivation in leukemia cells

Kazuma Ohyashiki, Junko H. Ohyashiki, Hiroshi Iwama, Shigefumi Hayashi, Jerry W. Shay, Keisuke Toyama

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

We utilized fluorescent-labeled primers and modified the telomeric repeat amplification protocol (TRAP) to assess telomerase activity during the process of in vitro establishment in four different leukemia cell lines. We demonstrate that three of four leukemia cell lines had elevated telomerase activity, shortened telomeres, and the appearance of either dicentric chromosomes or acentric fragments. By contrast, one leukemia cell line (HAL-01) had high levels of telomerase activity, stable telomeres, but no obvious additional cytogenetic rearrangements. These experiments indicate that there may be several pathways involving telomerase activity in the establishment of leukemia cell lines.

Original languageEnglish (US)
Pages (from-to)417-421
Number of pages5
JournalInternational journal of oncology
Volume8
Issue number3
StatePublished - Mar 1 1996

Keywords

  • Chromosome instability
  • Leukemia
  • Telomerase activity
  • Telomere

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Ohyashiki, K., Ohyashiki, J. H., Iwama, H., Hayashi, S., Shay, J. W., & Toyama, K. (1996). Telomerase reactivation in leukemia cells. International journal of oncology, 8(3), 417-421.