Telomerase reactivation in leukemia cells

Kazuma Ohyashiki, Junko H. Ohyashiki, Hiroshi Iwama, Shigefumi Hayashi, Jerry W. Shay, Keisuke Toyama

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

We utilized fluorescent-labeled primers and modified the telomeric repeat amplification protocol (TRAP) to assess telomerase activity during the process of in vitro establishment in four different leukemia cell lines. We demonstrate that three of four leukemia cell lines had elevated telomerase activity, shortened telomeres, and the appearance of either dicentric chromosomes or acentric fragments. By contrast, one leukemia cell line (HAL-01) had high levels of telomerase activity, stable telomeres, but no obvious additional cytogenetic rearrangements. These experiments indicate that there may be several pathways involving telomerase activity in the establishment of leukemia cell lines.

Original languageEnglish (US)
Pages (from-to)417-421
Number of pages5
JournalInternational Journal of Oncology
Volume8
Issue number3
StatePublished - Mar 1996

Fingerprint

Telomerase
Leukemia
Cell Line
Telomere Shortening
Telomere
Cytogenetics
Chromosomes

Keywords

  • Chromosome instability
  • Leukemia
  • Telomerase activity
  • Telomere

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Ohyashiki, K., Ohyashiki, J. H., Iwama, H., Hayashi, S., Shay, J. W., & Toyama, K. (1996). Telomerase reactivation in leukemia cells. International Journal of Oncology, 8(3), 417-421.

Telomerase reactivation in leukemia cells. / Ohyashiki, Kazuma; Ohyashiki, Junko H.; Iwama, Hiroshi; Hayashi, Shigefumi; Shay, Jerry W.; Toyama, Keisuke.

In: International Journal of Oncology, Vol. 8, No. 3, 03.1996, p. 417-421.

Research output: Contribution to journalArticle

Ohyashiki, K, Ohyashiki, JH, Iwama, H, Hayashi, S, Shay, JW & Toyama, K 1996, 'Telomerase reactivation in leukemia cells', International Journal of Oncology, vol. 8, no. 3, pp. 417-421.
Ohyashiki K, Ohyashiki JH, Iwama H, Hayashi S, Shay JW, Toyama K. Telomerase reactivation in leukemia cells. International Journal of Oncology. 1996 Mar;8(3):417-421.
Ohyashiki, Kazuma ; Ohyashiki, Junko H. ; Iwama, Hiroshi ; Hayashi, Shigefumi ; Shay, Jerry W. ; Toyama, Keisuke. / Telomerase reactivation in leukemia cells. In: International Journal of Oncology. 1996 ; Vol. 8, No. 3. pp. 417-421.
@article{5200c24808e84bcebdaa656b300aa2a3,
title = "Telomerase reactivation in leukemia cells",
abstract = "We utilized fluorescent-labeled primers and modified the telomeric repeat amplification protocol (TRAP) to assess telomerase activity during the process of in vitro establishment in four different leukemia cell lines. We demonstrate that three of four leukemia cell lines had elevated telomerase activity, shortened telomeres, and the appearance of either dicentric chromosomes or acentric fragments. By contrast, one leukemia cell line (HAL-01) had high levels of telomerase activity, stable telomeres, but no obvious additional cytogenetic rearrangements. These experiments indicate that there may be several pathways involving telomerase activity in the establishment of leukemia cell lines.",
keywords = "Chromosome instability, Leukemia, Telomerase activity, Telomere",
author = "Kazuma Ohyashiki and Ohyashiki, {Junko H.} and Hiroshi Iwama and Shigefumi Hayashi and Shay, {Jerry W.} and Keisuke Toyama",
year = "1996",
month = "3",
language = "English (US)",
volume = "8",
pages = "417--421",
journal = "International Journal of Oncology",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "3",

}

TY - JOUR

T1 - Telomerase reactivation in leukemia cells

AU - Ohyashiki, Kazuma

AU - Ohyashiki, Junko H.

AU - Iwama, Hiroshi

AU - Hayashi, Shigefumi

AU - Shay, Jerry W.

AU - Toyama, Keisuke

PY - 1996/3

Y1 - 1996/3

N2 - We utilized fluorescent-labeled primers and modified the telomeric repeat amplification protocol (TRAP) to assess telomerase activity during the process of in vitro establishment in four different leukemia cell lines. We demonstrate that three of four leukemia cell lines had elevated telomerase activity, shortened telomeres, and the appearance of either dicentric chromosomes or acentric fragments. By contrast, one leukemia cell line (HAL-01) had high levels of telomerase activity, stable telomeres, but no obvious additional cytogenetic rearrangements. These experiments indicate that there may be several pathways involving telomerase activity in the establishment of leukemia cell lines.

AB - We utilized fluorescent-labeled primers and modified the telomeric repeat amplification protocol (TRAP) to assess telomerase activity during the process of in vitro establishment in four different leukemia cell lines. We demonstrate that three of four leukemia cell lines had elevated telomerase activity, shortened telomeres, and the appearance of either dicentric chromosomes or acentric fragments. By contrast, one leukemia cell line (HAL-01) had high levels of telomerase activity, stable telomeres, but no obvious additional cytogenetic rearrangements. These experiments indicate that there may be several pathways involving telomerase activity in the establishment of leukemia cell lines.

KW - Chromosome instability

KW - Leukemia

KW - Telomerase activity

KW - Telomere

UR - http://www.scopus.com/inward/record.url?scp=0029946049&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029946049&partnerID=8YFLogxK

M3 - Article

VL - 8

SP - 417

EP - 421

JO - International Journal of Oncology

JF - International Journal of Oncology

SN - 1019-6439

IS - 3

ER -