Telomerase recruitment in Saccharomyces cerevisiae is not dependent on Tel1-mediated phosphorylation of Cdc13

Hua Gao, Tasha B. Toro, Margherita Paschini, Bari Braunstein-Ballew, Rachel B. Cervantes, Victoria Lundblad

Research output: Contribution to journalArticle

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Abstract

In Saccharomyces cerevisiae, association between the Est1 telomerase subunit and the telomere-binding protein Cdc13 is essential for telomerase to be recruited to its site of action. A current model proposes that Tel1 binding to telomeres marks them for elongation, as the result of phosphorylation of a proposed S/TQ cluster in the telomerase recruitment domain of Cdc13. However, three observations presented here argue against one key aspect of this model. First, the pattern of Cdc13 phosphatase-sensitive isoforms is not altered by loss of Tel1 function or by mutations introduced into two conserved serines (S249 and S255) in the Cdc13 recruitment domain. Second, an interaction between Cdc13 and Est1, as monitored by a two-hybrid assay, is dependent on S255 but Tel1-independent. Finally, a derivative of Cdc13, cdc13-(S/TQ) 11→(S/TA)11, in which every potential consensus phosphorylation site for Tel1 has been eliminated, confers nearly wild-type telomere length. These results are inconsistent with a model in which the Cdc13-Est1 interaction is regulated by Tel1-mediated phosphorylation of the Cdc13 telomerase recruitment domain. We propose an alternative model for the role of Tel1 in telomere homeostasis, which is based on the assumption that Tel1 performs the same molecular task at double-strand breaks (DSBs) and chromosome termini.

Original languageEnglish (US)
Pages (from-to)1147-1159
Number of pages13
JournalGenetics
Volume186
Issue number4
DOIs
StatePublished - Dec 2010

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Telomerase
Saccharomyces cerevisiae
Phosphorylation
Telomere
Telomere-Binding Proteins
Telomere Homeostasis
Chromosome Breakage
Two-Hybrid System Techniques
Phosphoric Monoester Hydrolases
Serine
Protein Isoforms
Mutation

ASJC Scopus subject areas

  • Genetics

Cite this

Gao, H., Toro, T. B., Paschini, M., Braunstein-Ballew, B., Cervantes, R. B., & Lundblad, V. (2010). Telomerase recruitment in Saccharomyces cerevisiae is not dependent on Tel1-mediated phosphorylation of Cdc13. Genetics, 186(4), 1147-1159. https://doi.org/10.1534/genetics.110.122044

Telomerase recruitment in Saccharomyces cerevisiae is not dependent on Tel1-mediated phosphorylation of Cdc13. / Gao, Hua; Toro, Tasha B.; Paschini, Margherita; Braunstein-Ballew, Bari; Cervantes, Rachel B.; Lundblad, Victoria.

In: Genetics, Vol. 186, No. 4, 12.2010, p. 1147-1159.

Research output: Contribution to journalArticle

Gao, H, Toro, TB, Paschini, M, Braunstein-Ballew, B, Cervantes, RB & Lundblad, V 2010, 'Telomerase recruitment in Saccharomyces cerevisiae is not dependent on Tel1-mediated phosphorylation of Cdc13', Genetics, vol. 186, no. 4, pp. 1147-1159. https://doi.org/10.1534/genetics.110.122044
Gao, Hua ; Toro, Tasha B. ; Paschini, Margherita ; Braunstein-Ballew, Bari ; Cervantes, Rachel B. ; Lundblad, Victoria. / Telomerase recruitment in Saccharomyces cerevisiae is not dependent on Tel1-mediated phosphorylation of Cdc13. In: Genetics. 2010 ; Vol. 186, No. 4. pp. 1147-1159.
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