Telomere G-rich overhang length measurement: DSN method

Yong Zhao, Jerry W. Shay, Woodring E. Wright

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Telomeres terminate in 3′ single-stranded G-rich overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex specific nuclease (DSN) to digest all double-stranded DNA including telomeres to fragments of <10 bp, leaving the single-stranded overhangs intact. Their length can then be determined by Southern blot using super-sensitive telomere C-rich probes.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages55-62
Number of pages8
Volume1587
DOIs
StatePublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1587
ISSN (Print)10643745

Keywords

  • Duplex specific nuclease (DSN)
  • G-rich overhang
  • Southern blot
  • Telomere
  • Telomere C-rich probe

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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