@inbook{53f064737e13426993ff6de38158bf83,
title = "Telomere G-rich overhang length measurement: DSN method",
abstract = "Telomeres terminate in 3′ single-stranded G-rich overhangs that function in telomere end protection and telomerase action. An accurate measurement of overhang length is challenging due to the presence of many kilobases of double-stranded telomere DNA. Here, a simple method is described that utilizes duplex specific nuclease (DSN) to digest all double-stranded DNA including telomeres to fragments of <10 bp, leaving the single-stranded overhangs intact. Their length can then be determined by Southern blot using super-sensitive telomere C-rich probes.",
keywords = "Duplex specific nuclease (DSN), G-rich overhang, Southern blot, Telomere, Telomere C-rich probe",
author = "Yong Zhao and Shay, {Jerry W.} and Wright, {Woodring E.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6892-3_5",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "55--62",
booktitle = "Methods in Molecular Biology",
}